Picoeukaryote counts determined from the community sampled at station West and East (PS97) before and after exposure to different Fe and Mn availabilities ...
Two Fe-Mn bottle amendment experiments with two natural phytoplankton communities were performed during Polarstern expedition PS97 in 2016 in the Drake Passage. At two locations, sea water was pumped (using trace metals clean techniques) from 25m depth and used to fill polycarbonate bottles after ha...
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Online Access: | https://dx.doi.org/10.1594/pangaea.935862 https://doi.pangaea.de/10.1594/PANGAEA.935862 |
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ftdatacite:10.1594/pangaea.935862 2024-09-09T19:09:26+00:00 Picoeukaryote counts determined from the community sampled at station West and East (PS97) before and after exposure to different Fe and Mn availabilities ... Balaguer, Jenna Koch, Florian Hassler, Christel S Trimborn, Scarlett 2021 text/tab-separated-values https://dx.doi.org/10.1594/pangaea.935862 https://doi.pangaea.de/10.1594/PANGAEA.935862 en eng PANGAEA https://dx.doi.org/10.1594/pangaea.935868 https://dx.doi.org/10.1038/s42003-022-03148-8 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 co-limitation Phytoplankton composition Southern Ocean trace elements Event label Experiment Experimental treatment Incubation duration Picoeukaryotes Membrane pump Flow cytometry PS97 Polarstern Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas SPP1158 dataset Dataset 2021 ftdatacite https://doi.org/10.1594/pangaea.93586210.1594/pangaea.93586810.1038/s42003-022-03148-8 2024-06-17T09:38:37Z Two Fe-Mn bottle amendment experiments with two natural phytoplankton communities were performed during Polarstern expedition PS97 in 2016 in the Drake Passage. At two locations, sea water was pumped (using trace metals clean techniques) from 25m depth and used to fill polycarbonate bottles after having passed through a cleaned 200 μm mesh (removing large grazers). The Control treatment was the sampled seawater without any trace metals addition while the other three treatments were enriched with either FeCl3 alone (0.5 nM; +Fe treatment) or MnCl2 alone (1 nM; +Mn treatment) or both trace metals together (+FeMn treatment). All treatments were done in triplicate 2,5L PC bottles. All incubation bottles were maintained at 30 μmol photons m-2 s-1 under a 16:8 (light:dark) hour cycle at 1 ̊C.Autotrophic picoeukaryotes were analyzed via flow cytometry. At the start and the end of the experiments, samples were preserved with 10% buffered formalin, flash-frozen in liquid nitrogen, and analyzed flow cytometrically to ... Dataset Antarc* Antarctic Arctic Drake Passage Phytoplankton Sea ice Southern Ocean DataCite Antarctic Arctic Drake Passage Southern Ocean |
institution |
Open Polar |
collection |
DataCite |
op_collection_id |
ftdatacite |
language |
English |
topic |
co-limitation Phytoplankton composition Southern Ocean trace elements Event label Experiment Experimental treatment Incubation duration Picoeukaryotes Membrane pump Flow cytometry PS97 Polarstern Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas SPP1158 |
spellingShingle |
co-limitation Phytoplankton composition Southern Ocean trace elements Event label Experiment Experimental treatment Incubation duration Picoeukaryotes Membrane pump Flow cytometry PS97 Polarstern Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas SPP1158 Balaguer, Jenna Koch, Florian Hassler, Christel S Trimborn, Scarlett Picoeukaryote counts determined from the community sampled at station West and East (PS97) before and after exposure to different Fe and Mn availabilities ... |
topic_facet |
co-limitation Phytoplankton composition Southern Ocean trace elements Event label Experiment Experimental treatment Incubation duration Picoeukaryotes Membrane pump Flow cytometry PS97 Polarstern Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas SPP1158 |
description |
Two Fe-Mn bottle amendment experiments with two natural phytoplankton communities were performed during Polarstern expedition PS97 in 2016 in the Drake Passage. At two locations, sea water was pumped (using trace metals clean techniques) from 25m depth and used to fill polycarbonate bottles after having passed through a cleaned 200 μm mesh (removing large grazers). The Control treatment was the sampled seawater without any trace metals addition while the other three treatments were enriched with either FeCl3 alone (0.5 nM; +Fe treatment) or MnCl2 alone (1 nM; +Mn treatment) or both trace metals together (+FeMn treatment). All treatments were done in triplicate 2,5L PC bottles. All incubation bottles were maintained at 30 μmol photons m-2 s-1 under a 16:8 (light:dark) hour cycle at 1 ̊C.Autotrophic picoeukaryotes were analyzed via flow cytometry. At the start and the end of the experiments, samples were preserved with 10% buffered formalin, flash-frozen in liquid nitrogen, and analyzed flow cytometrically to ... |
format |
Dataset |
author |
Balaguer, Jenna Koch, Florian Hassler, Christel S Trimborn, Scarlett |
author_facet |
Balaguer, Jenna Koch, Florian Hassler, Christel S Trimborn, Scarlett |
author_sort |
Balaguer, Jenna |
title |
Picoeukaryote counts determined from the community sampled at station West and East (PS97) before and after exposure to different Fe and Mn availabilities ... |
title_short |
Picoeukaryote counts determined from the community sampled at station West and East (PS97) before and after exposure to different Fe and Mn availabilities ... |
title_full |
Picoeukaryote counts determined from the community sampled at station West and East (PS97) before and after exposure to different Fe and Mn availabilities ... |
title_fullStr |
Picoeukaryote counts determined from the community sampled at station West and East (PS97) before and after exposure to different Fe and Mn availabilities ... |
title_full_unstemmed |
Picoeukaryote counts determined from the community sampled at station West and East (PS97) before and after exposure to different Fe and Mn availabilities ... |
title_sort |
picoeukaryote counts determined from the community sampled at station west and east (ps97) before and after exposure to different fe and mn availabilities ... |
publisher |
PANGAEA |
publishDate |
2021 |
url |
https://dx.doi.org/10.1594/pangaea.935862 https://doi.pangaea.de/10.1594/PANGAEA.935862 |
geographic |
Antarctic Arctic Drake Passage Southern Ocean |
geographic_facet |
Antarctic Arctic Drake Passage Southern Ocean |
genre |
Antarc* Antarctic Arctic Drake Passage Phytoplankton Sea ice Southern Ocean |
genre_facet |
Antarc* Antarctic Arctic Drake Passage Phytoplankton Sea ice Southern Ocean |
op_relation |
https://dx.doi.org/10.1594/pangaea.935868 https://dx.doi.org/10.1038/s42003-022-03148-8 |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_doi |
https://doi.org/10.1594/pangaea.93586210.1594/pangaea.93586810.1038/s42003-022-03148-8 |
_version_ |
1809823698462441472 |