Seawater carbonate chemistry and acid–base balance in the hæmolymph of European abalone (Haliotis tuberculata)

• Main Authors: Auzoux-Bordenave, Stephanie, Chevret, Sandra, Badou, Aïcha, Martin, Sophie, Di Giglio, Sarah, Dubois, Philippe
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2021
• Subjects: Acid-base regulation; Animalia; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria 20-1000 L or < 1 m**2; Haliotis tuberculata; Laboratory experiment; Mollusca; North Atlantic; Single species; Temperate; Type; Species; Registration number of species; Uniform resource locator/link to reference; Experiment; Experiment duration; Treatment; Salinity; Salinity, standard deviation; pH; pH, standard deviation; Temperature, water; Temperature, water, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Bicarbonate ion; Bicarbonate ion, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Aragonite saturation state; Aragonite saturation state, standard deviation; Calcite saturation state; Calcite saturation state, standard deviation; Haemolymph, pH; Haemolymph, pH, standard deviation; Haemolymph, temperature; Haemolymph, temperature, standard deviation; Haemolymph, alkalinity, total; Haemolymph, alkalinity, total, standard deviation; Haemolymph, partial pressure of carbon dioxide; Haemolymph, partial pressure of carbon dioxide, standard deviation; Haemolymph, total dissolved inorganic carbon; Haemolymph, total dissolved inorganic carbon, standard deviation; Haemolymph, bicarbonate ion; Haemolymph, bicarbonate ion, standard deviation; Haemolymph, carbonate ion; Haemolymph, carbonate ion, standard deviation; Haemolymph, aragonite saturation state; Haemolymph, aragonite saturation state, standard deviation; Haemolymph, calcite saturation state; Haemolymph, calcite saturation state, standard deviation; Protein, total; Protein, total, standard deviation; Carbonate system computation flag; Carbon dioxide; Carbon dioxide, standard deviation; Fugacity of carbon dioxide water at sea surface temperature wet air; Fugacity of carbon dioxide in seawater, standard deviation; Potentiometric; Potentiometric titration; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Calculated using seacarb after Orr et al. 2018; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.932922; https://doi.pangaea.de/10.1594/PANGAEA.932922

Ocean acidification (OA) and the associated changes in seawater carbonate chemistry pose a threat to calcifying organisms. This is particularly serious for shelled molluscs, in which shell growth and microstructure has been shown to be highly sensitive to OA. To improve our understanding of the responses of abalone to OA, this study investigated the effects of CO2-induced ocean acidification on extra-cellular acid–base parameters in the European abalone Haliotis tuberculata. Three-year-old adult abalone were exposed for 15 days to three different pH levels (7.9, 7.7, 7.4) representing current and predicted near-future conditions. Hæmolymph pH and total alkalinity were measured at different time points during exposure and used to calculate the carbonate parameters of the extracellular fluid. Total protein content was also measured to determine whether seawater acidification influences the composition and buffer capacity of hæmolymph. Extracellular pH was maintained at seawater pH 7.7 indicating that abalones are able to buffer moderate acidification (−0.2 pH units). This was not due to an accumulation of HCO3− ions but rather to a high hæmolymph protein concentration. By contrast, hæmolymph pH was significantly decreased after 5 days of exposure to pH 7.4, indicating that abalone do not compensate for higher decreases in seawater pH. Total alkalinity and dissolved inorganic carbon were also significantly decreased after 15 days of low pH exposure. It is concluded that changes in the acid–base balance of the hæmolymph might be involved in deleterious effects recorded in adult H. tuberculata facing severe OA stress. This would impact both the ecology and aquaculture of this commercially important species. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2021-06-22.