Seawater carbonate chemistry and pH of the extracellular calcifying fluid and skeletal concentrations of trace metals of three coral species (Pocillopora eydouxi, Porites lobata, and Porites rus)

• Main Authors: Shore, A, Day, R D, Stewart, J A, Burge, Colleen A
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2021
• Subjects: Acid-base regulation; Animalia; Benthic animals; Benthos; Biomass/Abundance/Elemental composition; Cnidaria; CO2 vent; Coast and continental shelf; Community composition and diversity; Entire community; Field observation; North Pacific; Pocillopora eydouxi; Porites lobata; Porites rus; Rocky-shore community; Single species; Tropical; Type; Identification; Species; Registration number of species; Uniform resource locator/link to reference; Site; δ11B; pH; pH, standard error; Calcium; Lithium/Calcium ratio; Barium/Calcium ratio; Aluminium/Calcium ratio; Phosphorus/Calcium ratio; Vanadium/Calcium ratio; Chromium/Calcium ratio; Manganese/Calcium ratio; Cobalt/Calcium ratio; Nickel/Calcium ratio; Copper/Calcium ratio; Zinc/Calcium ratio; Rubidium/Calcium ratio; Molybdenum/Calcium ratio; Argentum/Calcium ratio; Cadmium/Calcium ratio; Tin/Calcium ratio; Antimony/Calcium ratio; Caesium/Calcium ratio; Neodymium/Calcium ratio; Tungsten/Calcium ratio; Lead/Calcium ratio; Uranium/Calcium ratio; Sodium/Calcium ratio; Magnesium/Calcium ratio; Iron/Calcium ratio; Strontium/Calcium ratio; Lithium/Magnesium ratio; Replicates; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide water at sea surface temperature wet air, standard error; Number of sequences; Number of sequences, standard error; Species richness; Species richness, standard error; Shannon Diversity Index; Shannon Diversity index, standard error; Temperature, water; Salinity; Alkalinity, total; Carbon, inorganic, dissolved; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Aragonite saturation state; Calcite saturation state; Experiment; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Pacific; Rus’; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.930632; https://doi.pangaea.de/10.1594/PANGAEA.930632

Ocean acidification (OA) threatens the growth and function of coral reef ecosystems. A key component to coral health is the microbiome, but little is known about the impact of OA on coral microbiomes. A submarine CO2 vent at Maug Island in the Northern Marianas Islands provides a natural pH gradient to investigate coral responses to long-term OA conditions. Three coral species (Pocillopora eydouxi, Porites lobata, and Porites rus) were sampled from three sites where mean seawater pH is 8.04, 7.98, and 7.94. We characterized coral bacterial communities (using 16S rRNA gene sequencing) and determined pH of the extracellular calcifying fluid (ECF) (using skeletal boron isotopes) across the seawater pH gradient. Bacterial communities of both Porites species stabilized (decreases in community dispersion) with decreased seawater pH, coupled with large increases in the abundance of Endozoicomonas, an endosymbiont. P. lobata experienced a significant decrease in ECF pH near the vent, whereas P. rus experienced a trending decrease in ECF pH near the vent. By contrast, Pocillopora exhibited bacterial community destabilization (increases in community dispersion), with significant decreases in Endozoicomonas abundance, while its ECF pH remained unchanged across the pH gradient. Our study shows that OA has multiple consequences on Endozoicomonas abundance and suggests that Endozoicomonas abundance may be an indicator of coral response to OA. We reveal an interesting dichotomy between two facets of coral physiology (regulation of bacterial communities and regulation of calcification), highlighting the importance of multidisciplinary approaches to understanding coral health and function in a changing ocean. : For trace metal parameters, value 0 stands for value < limit of detection. In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2021-04-19.