Seawater carbonate chemistry and enzymatic antioxidant defense, protein repair and removal of temperate shark (Scyliorhinus canicula)

• Main Authors: Pegado, Maria, Santos, Catarina P, Pimentel, Marta, Cyrne, Ricardo, Sampaio, Eduardo, Temporão, Ana, Röckner, Janina, Diniz, Mário, Rosa, Rui
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2020
• Subjects: Animalia; Benthos; Chordata; Coast and continental shelf; Containers and aquaria 20-1000 L or < 1 m**2; Laboratory experiment; Nekton; North Atlantic; Other metabolic rates; Other studied parameter or process; Scyliorhinus canicula; Single species; Temperate; Type; Species; Registration number of species; Uniform resource locator/link to reference; Identification; Experiment; Treatment; Replicate; Organ; Catalase activity, per protein mass; Total glutathione peroxidases activity, per protein mass; Ubiquitin; DNA damage, per protein; Lipid peroxidation, per wet mass; Superoxide dismutase activity, inhibition, per protein; Heat shock protein, per protein mass; Temperature, water; Temperature, water, standard deviation; pH; pH, standard deviation; Salinity; Salinity, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Bicarbonate ion; Bicarbonate ion, standard deviation; Aragonite saturation state; Aragonite saturation state, standard deviation; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Carbonate ion; Carbon, inorganic, dissolved; Calcite saturation state; Calculated; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Canicula; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.929859; https://doi.pangaea.de/10.1594/PANGAEA.929859

Ocean acidification is a consequence of chemical changes driven mainly by a continuous uptake of carbon dioxide, resulting in pH decrease. This phenomenon represents an additional threat to marine life, with expected effects ranging from changes in behavioral responses and calcification rates to the potential promotion of oxidative stress. To unravel the impacts of ocean acidification on the antioxidant system of sharks, we performed a long-term exposure (9 months, since early embryogenesis) to high CO2 conditions (pCO2 900 μatm) on a temperate shark (Scyliorhinus canicula). The following biomarkers were measured: enzymatic antioxidant defense (superoxide dismutase, catalase and glutathione peroxidase), protein repair and removal (heat shock proteins and ubiquitin), and oxidative damage on lipids (malondialdehyde) and DNA (8-hydroxy-2′-deoxyguanosine). Changes in the antioxidant enzyme defense were restricted to an increase in catalase activity in the muscle, an enzyme that plays a major role in oxidative stress mitigation. On the other hand, no evidence of oxidative damage was found, indicating that the observed increase in catalase activity may be enough to neutralize the effects of potentially higher reactive oxygen species. These results further indicate that these sharks' antioxidant system can successfully cope with the levels of carbon dioxide projected for the end of the century. Nonetheless, the interaction between ocean acidification and the rise in temperature expected to occur in a near future may disturb their antioxidant capacity, requiring further investigation. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2021-03-23.