Seawater carbonate chemistry and growth and physiological, biochemical, cellular, behavioural and reproductive responses of the sea urchin Paracentrotus lividus

• Main Authors: Marceta, T, Matozzo, V, Alban, Silvia, Badocco, D, Pastore, P, Marin, Maria Gabriella
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2020
• Subjects: Animalia; Behaviour; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria 20-1000 L or < 1 m**2; Echinodermata; Laboratory experiment; Mediterranean Sea; Other metabolic rates; Paracentrotus lividus; Reproduction; FOS Medical biotechnology; Respiration; Single species; Temperate; Type; Species; Registration number of species; Uniform resource locator/link to reference; Treatment; Sex; Day of experiment; Identification; Respiration rate, oxygen; Ammonia excretion; Assimilation efficiency; Righting time; Gonadosomatic index; Superoxide dismutase activity, unit per protein mass; Catalase activity, unit per protein mass; Total coelomocyte count; Coelomocyte volume; Lysozyme activity, per protein mass; Temperature, water; Temperature, water, standard deviation; Salinity; Salinity, standard deviation; pH; pH, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Calcite saturation state; Calcite saturation state, standard deviation; Aragonite saturation state; Aragonite saturation state, standard deviation; Carbonate system computation flag; Carbon dioxide; Carbon dioxide, standard deviation; Fugacity of carbon dioxide water at sea surface temperature wet air; Fugacity of carbon dioxide in seawater, standard deviation; Bicarbonate ion; Bicarbonate ion, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Potentiometric titration; Calculated using seacarb after Nisumaa et al. 2010; Calculated using seacarb after Orr et al. 2018; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.929633; https://doi.pangaea.de/10.1594/PANGAEA.929633

Seawater pH lowering, known as ocean acidification, is considered among the major threats to marine environment. In this study, post-spawning adults of the sea urchin Paracentrotus lividus were maintained at three pH values (8.0, 7.7, 7.4) for 60 days. Physiological, biochemical, cellular, behavioural and reproductive responses were evaluated in males and females. Significant differences between sexes were observed, with higher ammonia excretion and lower catalase activity in males. Respiration rate (after 21 days), catalase activity in gonads and total coelomocyte count showed the same increasing trend in males and females under low pH. Ammonia excretion, gonadosomatic index and lysozyme activity exhibited opposite responses to low pH, with an increasing trend in males and decreasing in females. Results demonstrated that exposure to low pH could result in different response strategies of male and female sea urchins at a physiological, biochemical and immunological level. Reduced female gonadosomatic index under low pH suggested decreased energy investment in reproduction. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2021-03-24.