Seawater carbonate chemistry and growth and physiology of the marine diatom Thalassiosira pseudonana (Coscinodiscophyceae) ...

• Main Authors: Laws, Edward A, McClellan, S Alex
• Format: Dataset
• Language: English
• Published: PANGAEA 2020
• Subjects: Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria <20 L; Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; Light; Macro-nutrients; Not applicable; Ochrophyta; Pelagos; Phytoplankton; Primary production/Photosynthesis; Respiration; Single species; Temperature; Thalassiosira pseudonana; Type; Species; Registration number of species; Uniform resource locator/link to reference; DATE/TIME; Temperature, water; Treatment; Irradiance; Partial pressure of carbon dioxide water at sea surface temperature wet air; Growth rate; Growth rate, standard deviation; Ratio; Productivity index carbon/chlorophyll a; Maximum quantum yield of photosystem II; Maximum photosynthetic rate carbon/chlorophyll a; Maximum photosynthetic rate carbon/chlorophyll a, standard error; Initial slope of the photosynthesis-irradiance curve; Initial slope of the photosynthesis-irradiance curve, standard error; Carbon/Nitrogen ratio; Carbon/Chlorophyll a ratio; Nitrogen/chlorophyll a ratio; Salinity; Alkalinity, total; pH; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Carbon, inorganic, dissolved; Aragonite saturation state; Calcite saturation state; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.929551; https://doi.pangaea.de/10.1594/PANGAEA.929551

The marine diatom Thalassiosira pseudonana was grown in continuous culture systems to study the interactive effects of temperature, irradiance, nutrient limitation, and the partial pressure of CO2 (pCO2) on its growth and physiological characteristics. The cells were able to grow at all combinations of low and high irradiance (50 and 300 μmol photons/m**2/ s, respectively, of visible light), low and high pCO2 (400 and 1,000 μatm, respectively), nutrient limitation (nitrate‐limited and nutrient‐replete conditions), and temperatures of 10–32°C. Under nutrient‐replete conditions, there was no adverse effect of high pCO2 on growth rates at temperatures of 10–25°C. The response of the cells to high pCO2 was similar at low and high irradiance. At supraoptimal temperatures of 30°C or higher, high pCO2 depressed growth rates at both low and high irradiance. Under nitrate‐limited conditions, cells were grown at 38 ± 2.4% of their nutrient‐saturated rates at the same temperature, irradiance, and pCO2. Dark respiration ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2021-03-23. ...