Seawater carbonate chemistry and half-time of calcein influx in the coral Stylophora pistillata ...
Coral calcification relies on the transport of ions and molecules to the extracellular calcifying medium (ECM). Little is known about paracellular transport (via intercellular junctions) in corals and other marine calcifiers. Here, we investigated whether the permeability of the paracellular pathway...
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ftdatacite:10.1594/pangaea.929464 2024-02-27T08:44:14+00:00 Seawater carbonate chemistry and half-time of calcein influx in the coral Stylophora pistillata ... Venn, Alexander A Bernardet, Coralie Chabenat, Apolline Tambutté, Eric Tambutté, Sylvie 2020 text/tab-separated-values https://dx.doi.org/10.1594/pangaea.929464 https://doi.pangaea.de/10.1594/PANGAEA.929464 en eng PANGAEA https://cran.r-project.org/web/packages/seacarb/index.html https://dx.doi.org/10.1242/jeb.227074 https://dx.doi.org/10.1594/pangaea.918127 https://cran.r-project.org/web/packages/seacarb/index.html Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 Acid-base regulation Animalia Benthic animals Benthos Calcification/Dissolution Cnidaria Containers and aquaria 20-1000 L or < 1 m**2 Laboratory experiment Laboratory strains Not applicable Other studied parameter or process Single species Stylophora pistillata Type Species Registration number of species Uniform resource locator/link to reference Treatment Time in minutes pH, extracellular pH, intracellular Distance Comment Temperature, water Salinity Alkalinity, total pH Carbonate system computation flag Carbon dioxide Fugacity of carbon dioxide water at sea surface temperature wet air Partial pressure of carbon dioxide water at sea surface temperature wet air Bicarbonate ion Carbonate ion Carbon, inorganic, dissolved Aragonite saturation state Calcite saturation state Potentiometric titration Spectrophotometric Calculated using seacarb after Nisumaa et al. 2010 Ocean Acidification International Coordination Centre OA-ICC dataset Dataset 2020 ftdatacite https://doi.org/10.1594/pangaea.92946410.1242/jeb.22707410.1594/pangaea.918127 2024-02-01T15:26:49Z Coral calcification relies on the transport of ions and molecules to the extracellular calcifying medium (ECM). Little is known about paracellular transport (via intercellular junctions) in corals and other marine calcifiers. Here, we investigated whether the permeability of the paracellular pathway varied in different environmental conditions in the coral Stylophora pistillata. Using the fluorescent dye calcein, we characterised the dynamics of calcein influx from seawater to the ECM and showed that increases in paracellular permeability (leakiness) induced by hyperosmotic treatment could be detected by changes in calcein influx rates. We then used the calcein-imaging approach to investigate the effects of two environmental stressors on paracellular permeability: seawater acidification and temperature change. Under conditions of seawater acidification (pH 7.2) known to depress pH in the ECM and the calcifying cells of S. pistillata, we observed a decrease in half-times of calcein influx, indicating ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2021-03-15. ... Dataset Ocean acidification DataCite Metadata Store (German National Library of Science and Technology) |
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Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
English |
topic |
Acid-base regulation Animalia Benthic animals Benthos Calcification/Dissolution Cnidaria Containers and aquaria 20-1000 L or < 1 m**2 Laboratory experiment Laboratory strains Not applicable Other studied parameter or process Single species Stylophora pistillata Type Species Registration number of species Uniform resource locator/link to reference Treatment Time in minutes pH, extracellular pH, intracellular Distance Comment Temperature, water Salinity Alkalinity, total pH Carbonate system computation flag Carbon dioxide Fugacity of carbon dioxide water at sea surface temperature wet air Partial pressure of carbon dioxide water at sea surface temperature wet air Bicarbonate ion Carbonate ion Carbon, inorganic, dissolved Aragonite saturation state Calcite saturation state Potentiometric titration Spectrophotometric Calculated using seacarb after Nisumaa et al. 2010 Ocean Acidification International Coordination Centre OA-ICC |
spellingShingle |
Acid-base regulation Animalia Benthic animals Benthos Calcification/Dissolution Cnidaria Containers and aquaria 20-1000 L or < 1 m**2 Laboratory experiment Laboratory strains Not applicable Other studied parameter or process Single species Stylophora pistillata Type Species Registration number of species Uniform resource locator/link to reference Treatment Time in minutes pH, extracellular pH, intracellular Distance Comment Temperature, water Salinity Alkalinity, total pH Carbonate system computation flag Carbon dioxide Fugacity of carbon dioxide water at sea surface temperature wet air Partial pressure of carbon dioxide water at sea surface temperature wet air Bicarbonate ion Carbonate ion Carbon, inorganic, dissolved Aragonite saturation state Calcite saturation state Potentiometric titration Spectrophotometric Calculated using seacarb after Nisumaa et al. 2010 Ocean Acidification International Coordination Centre OA-ICC Venn, Alexander A Bernardet, Coralie Chabenat, Apolline Tambutté, Eric Tambutté, Sylvie Seawater carbonate chemistry and half-time of calcein influx in the coral Stylophora pistillata ... |
topic_facet |
Acid-base regulation Animalia Benthic animals Benthos Calcification/Dissolution Cnidaria Containers and aquaria 20-1000 L or < 1 m**2 Laboratory experiment Laboratory strains Not applicable Other studied parameter or process Single species Stylophora pistillata Type Species Registration number of species Uniform resource locator/link to reference Treatment Time in minutes pH, extracellular pH, intracellular Distance Comment Temperature, water Salinity Alkalinity, total pH Carbonate system computation flag Carbon dioxide Fugacity of carbon dioxide water at sea surface temperature wet air Partial pressure of carbon dioxide water at sea surface temperature wet air Bicarbonate ion Carbonate ion Carbon, inorganic, dissolved Aragonite saturation state Calcite saturation state Potentiometric titration Spectrophotometric Calculated using seacarb after Nisumaa et al. 2010 Ocean Acidification International Coordination Centre OA-ICC |
description |
Coral calcification relies on the transport of ions and molecules to the extracellular calcifying medium (ECM). Little is known about paracellular transport (via intercellular junctions) in corals and other marine calcifiers. Here, we investigated whether the permeability of the paracellular pathway varied in different environmental conditions in the coral Stylophora pistillata. Using the fluorescent dye calcein, we characterised the dynamics of calcein influx from seawater to the ECM and showed that increases in paracellular permeability (leakiness) induced by hyperosmotic treatment could be detected by changes in calcein influx rates. We then used the calcein-imaging approach to investigate the effects of two environmental stressors on paracellular permeability: seawater acidification and temperature change. Under conditions of seawater acidification (pH 7.2) known to depress pH in the ECM and the calcifying cells of S. pistillata, we observed a decrease in half-times of calcein influx, indicating ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2021-03-15. ... |
format |
Dataset |
author |
Venn, Alexander A Bernardet, Coralie Chabenat, Apolline Tambutté, Eric Tambutté, Sylvie |
author_facet |
Venn, Alexander A Bernardet, Coralie Chabenat, Apolline Tambutté, Eric Tambutté, Sylvie |
author_sort |
Venn, Alexander A |
title |
Seawater carbonate chemistry and half-time of calcein influx in the coral Stylophora pistillata ... |
title_short |
Seawater carbonate chemistry and half-time of calcein influx in the coral Stylophora pistillata ... |
title_full |
Seawater carbonate chemistry and half-time of calcein influx in the coral Stylophora pistillata ... |
title_fullStr |
Seawater carbonate chemistry and half-time of calcein influx in the coral Stylophora pistillata ... |
title_full_unstemmed |
Seawater carbonate chemistry and half-time of calcein influx in the coral Stylophora pistillata ... |
title_sort |
seawater carbonate chemistry and half-time of calcein influx in the coral stylophora pistillata ... |
publisher |
PANGAEA |
publishDate |
2020 |
url |
https://dx.doi.org/10.1594/pangaea.929464 https://doi.pangaea.de/10.1594/PANGAEA.929464 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
https://cran.r-project.org/web/packages/seacarb/index.html https://dx.doi.org/10.1242/jeb.227074 https://dx.doi.org/10.1594/pangaea.918127 https://cran.r-project.org/web/packages/seacarb/index.html |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_doi |
https://doi.org/10.1594/pangaea.92946410.1242/jeb.22707410.1594/pangaea.918127 |
_version_ |
1792052617741860864 |