Seawater carbonate chemistry and half-time of calcein influx in the coral Stylophora pistillata ...

• Main Authors: Venn, Alexander A, Bernardet, Coralie, Chabenat, Apolline, Tambutté, Eric, Tambutté, Sylvie
• Format: Dataset
• Language: English
• Published: PANGAEA 2020
• Subjects: Acid-base regulation; Animalia; Benthic animals; Benthos; Calcification/Dissolution; Cnidaria; Containers and aquaria 20-1000 L or < 1 m**2; Laboratory experiment; Laboratory strains; Not applicable; Other studied parameter or process; Single species; Stylophora pistillata; Type; Species; Registration number of species; Uniform resource locator/link to reference; Treatment; Time in minutes; pH, extracellular; pH, intracellular; Distance; Comment; Temperature, water; Salinity; Alkalinity, total; pH; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Carbon, inorganic, dissolved; Aragonite saturation state; Calcite saturation state; Potentiometric titration; Spectrophotometric; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.929464; https://doi.pangaea.de/10.1594/PANGAEA.929464

Coral calcification relies on the transport of ions and molecules to the extracellular calcifying medium (ECM). Little is known about paracellular transport (via intercellular junctions) in corals and other marine calcifiers. Here, we investigated whether the permeability of the paracellular pathway varied in different environmental conditions in the coral Stylophora pistillata. Using the fluorescent dye calcein, we characterised the dynamics of calcein influx from seawater to the ECM and showed that increases in paracellular permeability (leakiness) induced by hyperosmotic treatment could be detected by changes in calcein influx rates. We then used the calcein-imaging approach to investigate the effects of two environmental stressors on paracellular permeability: seawater acidification and temperature change. Under conditions of seawater acidification (pH 7.2) known to depress pH in the ECM and the calcifying cells of S. pistillata, we observed a decrease in half-times of calcein influx, indicating ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2021-03-15. ...