Seawater carbonate chemistry and polyspermy of a broadcast spawning bivalve ...

• Main Authors: Han, Yu, Shi, Wei, Tang, Y, Zhao, Xinguo, Du, Xueying, Sun, Shuge, Zhou, Weishang, Liu, Guangxu
• Format: Dataset
• Language: English
• Published: PANGAEA 2021
• Subjects: Animalia; Benthic animals; Benthos; Bottles or small containers/Aquaria <20 L; Coast and continental shelf; Laboratory experiment; Mollusca; North Pacific; Reproduction; FOS Medical biotechnology; Single species; Tegillarca granosa; Temperate; Type; Species; Registration number of species; Uniform resource locator/link to reference; Treatment; Polyspermy; Relative fluorescence intensity, ratio; Time in seconds; Fluorescence intensity; Microfilament migration; Adenosine triphosphate, per unit protein; Temperature, water; Temperature, water, standard deviation; Salinity; Salinity, standard deviation; pH; pH, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Aragonite saturation state; Aragonite saturation state, standard deviation; Calcite saturation state; Calcite saturation state, standard deviation; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Experiment; Potentiometric; Potentiometric titration; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Pacific; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.929201; https://doi.pangaea.de/10.1594/PANGAEA.929201

Ensuring that oocytes are fertilized by a single sperm during broadcast spawning is crucial for the fertilization success of many marine invertebrates. Although the adverse impacts of ocean acidification (OA) on various marine species have been revealed in recent years, its impact on polyspermy and the underlying mechanisms involved remain largely unknown. Therefore, in the present study, the effect of OA on polyspermy risk was assessed in a broadcast spawning bivalve, Tegillarca granosa. In addition, the impacts of OA on the two polyspermy blocking processes, the fast block (membrane depolarization) and the permanent block (cortical reaction), were investigated. The results show that the exposure of oocytes to two future OA scenarios (pH 7.8 and pH 7.4) leads to significant increases in polyspermy risk, about 1.70 and 2.38 times higher than the control, respectively. The maximum change in the membrane potential during oocyte membrane depolarization markedly decreased to 15.79% (pH 7.8) and 34.06% (pH 7.4) ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2021-03-15. ...