Seawater carbonate chemistry and CO2 acquisition efficiency and mitochondrial respiration in a coastal diatom
Diatom responses to ocean acidification have been documented with variable and controversial results. We grew the coastal diatom Thalassiosira weissflogii under 410 (LC, pH 8.13) vs 1000 μatm (HC, pH 7.83) pCO2 and at different levels of light (80, 140, 220 μmol photons/m**2/s), and found that light...
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Language: | English |
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PANGAEA - Data Publisher for Earth & Environmental Science
2021
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Online Access: | https://dx.doi.org/10.1594/pangaea.928761 https://doi.pangaea.de/10.1594/PANGAEA.928761 |
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Open Polar |
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DataCite Metadata Store (German National Library of Science and Technology) |
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English |
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Bottles or small containers/Aquaria <20 L Calcification/Dissolution Chromista Growth/Morphology Laboratory experiment Laboratory strains Light Not applicable Ochrophyta Pelagos Phytoplankton Primary production/Photosynthesis Respiration Single species Thalassiosira weissflogii Type Species Registration number of species Uniform resource locator/link to reference Treatment Growth rate Growth rate, standard deviation Cell size Cell size, standard deviation Chlorophyll a per cell Chlorophyll a, standard deviation Carotenoids per cell Carotenoids, standard deviation Carotenoids/Chlorophyll a ratio Carotenoids/Chlorophyll a ratio, standard deviation Maximum quantum yield of photosystem II Maximum quantum yield of photosystem II, standard deviation Electron transport rate, relative Electron transport rate, relative, standard deviation Non photochemical quenching Non photochemical quenching, standard deviation Maximal electron transport rate, relative Maximal electron transport rate, relative, standard deviation Electron transport rate efficiency Electron transport rate efficiency, standard deviation Light saturation point Light saturation point, standard deviation Effective quantum yield Effective quantum yield, standard deviation Carbon, inorganic, dissolved Carbon, inorganic, dissolved, standard deviation Ratio Ratio, standard deviation Net photosynthesis rate, oxygen, per cell Net photosynthesis rate, standard deviation Respiration rate, oxygen, per cell Respiration rate, oxygen, standard deviation Oxygen evolution, daytime Oxygen evolution, daytime, standard deviation Net photosynthesis rate, oxygen, per chlorophyll a Respiration rate, oxygen, per chlorophyll a Oxygen evolution per cell, daytime Extracellular carbonic anhydrase activity, per cell Extracellular carbonic anhydrase activity, standard deviation Time in days Salinity Temperature, water pH pH, standard deviation Alkalinity, total Alkalinity, total, standard deviation Bicarbonate ion Bicarbonate ion, standard deviation Carbonate ion Carbonate ion, standard deviation Carbon dioxide Carbon dioxide, standard deviation Carbonate system computation flag Fugacity of carbon dioxide water at sea surface temperature wet air Fugacity of carbon dioxide in seawater, standard deviation Partial pressure of carbon dioxide water at sea surface temperature wet air Partial pressure of carbon dioxide, standard deviation Aragonite saturation state Aragonite saturation state, standard deviation Calcite saturation state Calcite saturation state, standard deviation Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. 2010 Calculated using seacarb after Orr et al. 2018 Ocean Acidification International Coordination Centre OA-ICC |
spellingShingle |
Bottles or small containers/Aquaria <20 L Calcification/Dissolution Chromista Growth/Morphology Laboratory experiment Laboratory strains Light Not applicable Ochrophyta Pelagos Phytoplankton Primary production/Photosynthesis Respiration Single species Thalassiosira weissflogii Type Species Registration number of species Uniform resource locator/link to reference Treatment Growth rate Growth rate, standard deviation Cell size Cell size, standard deviation Chlorophyll a per cell Chlorophyll a, standard deviation Carotenoids per cell Carotenoids, standard deviation Carotenoids/Chlorophyll a ratio Carotenoids/Chlorophyll a ratio, standard deviation Maximum quantum yield of photosystem II Maximum quantum yield of photosystem II, standard deviation Electron transport rate, relative Electron transport rate, relative, standard deviation Non photochemical quenching Non photochemical quenching, standard deviation Maximal electron transport rate, relative Maximal electron transport rate, relative, standard deviation Electron transport rate efficiency Electron transport rate efficiency, standard deviation Light saturation point Light saturation point, standard deviation Effective quantum yield Effective quantum yield, standard deviation Carbon, inorganic, dissolved Carbon, inorganic, dissolved, standard deviation Ratio Ratio, standard deviation Net photosynthesis rate, oxygen, per cell Net photosynthesis rate, standard deviation Respiration rate, oxygen, per cell Respiration rate, oxygen, standard deviation Oxygen evolution, daytime Oxygen evolution, daytime, standard deviation Net photosynthesis rate, oxygen, per chlorophyll a Respiration rate, oxygen, per chlorophyll a Oxygen evolution per cell, daytime Extracellular carbonic anhydrase activity, per cell Extracellular carbonic anhydrase activity, standard deviation Time in days Salinity Temperature, water pH pH, standard deviation Alkalinity, total Alkalinity, total, standard deviation Bicarbonate ion Bicarbonate ion, standard deviation Carbonate ion Carbonate ion, standard deviation Carbon dioxide Carbon dioxide, standard deviation Carbonate system computation flag Fugacity of carbon dioxide water at sea surface temperature wet air Fugacity of carbon dioxide in seawater, standard deviation Partial pressure of carbon dioxide water at sea surface temperature wet air Partial pressure of carbon dioxide, standard deviation Aragonite saturation state Aragonite saturation state, standard deviation Calcite saturation state Calcite saturation state, standard deviation Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. 2010 Calculated using seacarb after Orr et al. 2018 Ocean Acidification International Coordination Centre OA-ICC Qu, Liming Campbell, Douglas A Gao, Kunshan Seawater carbonate chemistry and CO2 acquisition efficiency and mitochondrial respiration in a coastal diatom |
topic_facet |
Bottles or small containers/Aquaria <20 L Calcification/Dissolution Chromista Growth/Morphology Laboratory experiment Laboratory strains Light Not applicable Ochrophyta Pelagos Phytoplankton Primary production/Photosynthesis Respiration Single species Thalassiosira weissflogii Type Species Registration number of species Uniform resource locator/link to reference Treatment Growth rate Growth rate, standard deviation Cell size Cell size, standard deviation Chlorophyll a per cell Chlorophyll a, standard deviation Carotenoids per cell Carotenoids, standard deviation Carotenoids/Chlorophyll a ratio Carotenoids/Chlorophyll a ratio, standard deviation Maximum quantum yield of photosystem II Maximum quantum yield of photosystem II, standard deviation Electron transport rate, relative Electron transport rate, relative, standard deviation Non photochemical quenching Non photochemical quenching, standard deviation Maximal electron transport rate, relative Maximal electron transport rate, relative, standard deviation Electron transport rate efficiency Electron transport rate efficiency, standard deviation Light saturation point Light saturation point, standard deviation Effective quantum yield Effective quantum yield, standard deviation Carbon, inorganic, dissolved Carbon, inorganic, dissolved, standard deviation Ratio Ratio, standard deviation Net photosynthesis rate, oxygen, per cell Net photosynthesis rate, standard deviation Respiration rate, oxygen, per cell Respiration rate, oxygen, standard deviation Oxygen evolution, daytime Oxygen evolution, daytime, standard deviation Net photosynthesis rate, oxygen, per chlorophyll a Respiration rate, oxygen, per chlorophyll a Oxygen evolution per cell, daytime Extracellular carbonic anhydrase activity, per cell Extracellular carbonic anhydrase activity, standard deviation Time in days Salinity Temperature, water pH pH, standard deviation Alkalinity, total Alkalinity, total, standard deviation Bicarbonate ion Bicarbonate ion, standard deviation Carbonate ion Carbonate ion, standard deviation Carbon dioxide Carbon dioxide, standard deviation Carbonate system computation flag Fugacity of carbon dioxide water at sea surface temperature wet air Fugacity of carbon dioxide in seawater, standard deviation Partial pressure of carbon dioxide water at sea surface temperature wet air Partial pressure of carbon dioxide, standard deviation Aragonite saturation state Aragonite saturation state, standard deviation Calcite saturation state Calcite saturation state, standard deviation Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. 2010 Calculated using seacarb after Orr et al. 2018 Ocean Acidification International Coordination Centre OA-ICC |
description |
Diatom responses to ocean acidification have been documented with variable and controversial results. We grew the coastal diatom Thalassiosira weissflogii under 410 (LC, pH 8.13) vs 1000 μatm (HC, pH 7.83) pCO2 and at different levels of light (80, 140, 220 μmol photons/m**2/s), and found that light level alters physiological responses to OA. CO2 concentrating mechanisms (CCMs) were down-regulated in the HC-grown cells across all the light levels, as reflected by lowered activity of the periplasmic carbonic anhydrase and decreased photosynthetic affinity for CO2 or dissolved inorganic carbon. The specific growth rate was, however, enhanced significantly by 9.2% only at the limiting low light level. These results indicate that rather than CO2 “fertilization”, the energy saved from down-regulation of CCMs promoted the growth rate of the diatom when light availability is low, in parallel with enhanced respiration under OA to cope with the acidic stress by providing extra energy. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2020) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2021-01-28. |
format |
Dataset |
author |
Qu, Liming Campbell, Douglas A Gao, Kunshan |
author_facet |
Qu, Liming Campbell, Douglas A Gao, Kunshan |
author_sort |
Qu, Liming |
title |
Seawater carbonate chemistry and CO2 acquisition efficiency and mitochondrial respiration in a coastal diatom |
title_short |
Seawater carbonate chemistry and CO2 acquisition efficiency and mitochondrial respiration in a coastal diatom |
title_full |
Seawater carbonate chemistry and CO2 acquisition efficiency and mitochondrial respiration in a coastal diatom |
title_fullStr |
Seawater carbonate chemistry and CO2 acquisition efficiency and mitochondrial respiration in a coastal diatom |
title_full_unstemmed |
Seawater carbonate chemistry and CO2 acquisition efficiency and mitochondrial respiration in a coastal diatom |
title_sort |
seawater carbonate chemistry and co2 acquisition efficiency and mitochondrial respiration in a coastal diatom |
publisher |
PANGAEA - Data Publisher for Earth & Environmental Science |
publishDate |
2021 |
url |
https://dx.doi.org/10.1594/pangaea.928761 https://doi.pangaea.de/10.1594/PANGAEA.928761 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
https://CRAN.R-project.org/package=seacarb https://dx.doi.org/10.1016/j.marpolbul.2021.112008 https://CRAN.R-project.org/package=seacarb |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1594/pangaea.928761 https://doi.org/10.1016/j.marpolbul.2021.112008 |
_version_ |
1766158160166387712 |
spelling |
ftdatacite:10.1594/pangaea.928761 2023-05-15T17:51:07+02:00 Seawater carbonate chemistry and CO2 acquisition efficiency and mitochondrial respiration in a coastal diatom Qu, Liming Campbell, Douglas A Gao, Kunshan 2021 text/tab-separated-values https://dx.doi.org/10.1594/pangaea.928761 https://doi.pangaea.de/10.1594/PANGAEA.928761 en eng PANGAEA - Data Publisher for Earth & Environmental Science https://CRAN.R-project.org/package=seacarb https://dx.doi.org/10.1016/j.marpolbul.2021.112008 https://CRAN.R-project.org/package=seacarb Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 CC-BY Bottles or small containers/Aquaria <20 L Calcification/Dissolution Chromista Growth/Morphology Laboratory experiment Laboratory strains Light Not applicable Ochrophyta Pelagos Phytoplankton Primary production/Photosynthesis Respiration Single species Thalassiosira weissflogii Type Species Registration number of species Uniform resource locator/link to reference Treatment Growth rate Growth rate, standard deviation Cell size Cell size, standard deviation Chlorophyll a per cell Chlorophyll a, standard deviation Carotenoids per cell Carotenoids, standard deviation Carotenoids/Chlorophyll a ratio Carotenoids/Chlorophyll a ratio, standard deviation Maximum quantum yield of photosystem II Maximum quantum yield of photosystem II, standard deviation Electron transport rate, relative Electron transport rate, relative, standard deviation Non photochemical quenching Non photochemical quenching, standard deviation Maximal electron transport rate, relative Maximal electron transport rate, relative, standard deviation Electron transport rate efficiency Electron transport rate efficiency, standard deviation Light saturation point Light saturation point, standard deviation Effective quantum yield Effective quantum yield, standard deviation Carbon, inorganic, dissolved Carbon, inorganic, dissolved, standard deviation Ratio Ratio, standard deviation Net photosynthesis rate, oxygen, per cell Net photosynthesis rate, standard deviation Respiration rate, oxygen, per cell Respiration rate, oxygen, standard deviation Oxygen evolution, daytime Oxygen evolution, daytime, standard deviation Net photosynthesis rate, oxygen, per chlorophyll a Respiration rate, oxygen, per chlorophyll a Oxygen evolution per cell, daytime Extracellular carbonic anhydrase activity, per cell Extracellular carbonic anhydrase activity, standard deviation Time in days Salinity Temperature, water pH pH, standard deviation Alkalinity, total Alkalinity, total, standard deviation Bicarbonate ion Bicarbonate ion, standard deviation Carbonate ion Carbonate ion, standard deviation Carbon dioxide Carbon dioxide, standard deviation Carbonate system computation flag Fugacity of carbon dioxide water at sea surface temperature wet air Fugacity of carbon dioxide in seawater, standard deviation Partial pressure of carbon dioxide water at sea surface temperature wet air Partial pressure of carbon dioxide, standard deviation Aragonite saturation state Aragonite saturation state, standard deviation Calcite saturation state Calcite saturation state, standard deviation Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. 2010 Calculated using seacarb after Orr et al. 2018 Ocean Acidification International Coordination Centre OA-ICC dataset Dataset 2021 ftdatacite https://doi.org/10.1594/pangaea.928761 https://doi.org/10.1016/j.marpolbul.2021.112008 2022-02-08T16:27:35Z Diatom responses to ocean acidification have been documented with variable and controversial results. We grew the coastal diatom Thalassiosira weissflogii under 410 (LC, pH 8.13) vs 1000 μatm (HC, pH 7.83) pCO2 and at different levels of light (80, 140, 220 μmol photons/m**2/s), and found that light level alters physiological responses to OA. CO2 concentrating mechanisms (CCMs) were down-regulated in the HC-grown cells across all the light levels, as reflected by lowered activity of the periplasmic carbonic anhydrase and decreased photosynthetic affinity for CO2 or dissolved inorganic carbon. The specific growth rate was, however, enhanced significantly by 9.2% only at the limiting low light level. These results indicate that rather than CO2 “fertilization”, the energy saved from down-regulation of CCMs promoted the growth rate of the diatom when light availability is low, in parallel with enhanced respiration under OA to cope with the acidic stress by providing extra energy. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2020) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2021-01-28. Dataset Ocean acidification DataCite Metadata Store (German National Library of Science and Technology) |