Seawater carbonate chemistry and CO2 acquisition efficiency and mitochondrial respiration in a coastal diatom

• Main Authors: Qu, Liming, Campbell, Douglas A, Gao, Kunshan
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2021
• Subjects: Bottles or small containers/Aquaria <20 L; Calcification/Dissolution; Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; Light; Not applicable; Ochrophyta; Pelagos; Phytoplankton; Primary production/Photosynthesis; Respiration; Single species; Thalassiosira weissflogii; Type; Species; Registration number of species; Uniform resource locator/link to reference; Treatment; Growth rate; Growth rate, standard deviation; Cell size; Cell size, standard deviation; Chlorophyll a per cell; Chlorophyll a, standard deviation; Carotenoids per cell; Carotenoids, standard deviation; Carotenoids/Chlorophyll a ratio; Carotenoids/Chlorophyll a ratio, standard deviation; Maximum quantum yield of photosystem II; Maximum quantum yield of photosystem II, standard deviation; Electron transport rate, relative; Electron transport rate, relative, standard deviation; Non photochemical quenching; Non photochemical quenching, standard deviation; Maximal electron transport rate, relative; Maximal electron transport rate, relative, standard deviation; Electron transport rate efficiency; Electron transport rate efficiency, standard deviation; Light saturation point; Light saturation point, standard deviation; Effective quantum yield; Effective quantum yield, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Ratio; Ratio, standard deviation; Net photosynthesis rate, oxygen, per cell; Net photosynthesis rate, standard deviation; Respiration rate, oxygen, per cell; Respiration rate, oxygen, standard deviation; Oxygen evolution, daytime; Oxygen evolution, daytime, standard deviation; Net photosynthesis rate, oxygen, per chlorophyll a; Respiration rate, oxygen, per chlorophyll a; Oxygen evolution per cell, daytime; Extracellular carbonic anhydrase activity, per cell; Extracellular carbonic anhydrase activity, standard deviation; Time in days; Salinity; Temperature, water; pH; pH, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Bicarbonate ion; Bicarbonate ion, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbon dioxide; Carbon dioxide, standard deviation; Carbonate system computation flag; Fugacity of carbon dioxide water at sea surface temperature wet air; Fugacity of carbon dioxide in seawater, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Aragonite saturation state; Aragonite saturation state, standard deviation; Calcite saturation state; Calcite saturation state, standard deviation; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Calculated using seacarb after Orr et al. 2018; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.928761; https://doi.pangaea.de/10.1594/PANGAEA.928761

Diatom responses to ocean acidification have been documented with variable and controversial results. We grew the coastal diatom Thalassiosira weissflogii under 410 (LC, pH 8.13) vs 1000 μatm (HC, pH 7.83) pCO2 and at different levels of light (80, 140, 220 μmol photons/m**2/s), and found that light level alters physiological responses to OA. CO2 concentrating mechanisms (CCMs) were down-regulated in the HC-grown cells across all the light levels, as reflected by lowered activity of the periplasmic carbonic anhydrase and decreased photosynthetic affinity for CO2 or dissolved inorganic carbon. The specific growth rate was, however, enhanced significantly by 9.2% only at the limiting low light level. These results indicate that rather than CO2 “fertilization”, the energy saved from down-regulation of CCMs promoted the growth rate of the diatom when light availability is low, in parallel with enhanced respiration under OA to cope with the acidic stress by providing extra energy. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2020) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2021-01-28.