Combined amino acids in aerosol samples from Ny-Alesund, Svalbard Island, 2015
Combined amino acids (CAA) were quantified in 7 aerosol samples collected during the Arctic campaign at Gruvebadet Laboratory in 2015. Combined amino acids (CAA) refer to amino acids contained in peptides, polipeptides and proteins.Aerosol samples were collected in quartz fiber filters. They were sp...
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PANGAEA - Data Publisher for Earth & Environmental Science
2021
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Online Access: | https://dx.doi.org/10.1594/pangaea.928097 https://doi.pangaea.de/10.1594/PANGAEA.928097 |
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ftdatacite:10.1594/pangaea.928097 2023-05-15T14:56:55+02:00 Combined amino acids in aerosol samples from Ny-Alesund, Svalbard Island, 2015 Feltracco, Matteo Barbaro, Elena Spolaor, Andrea Vecchiato, Marco Callegaro, Alice Burgay, Francois Vardè, Massimiliano Maffezzoli, Niccolò Dallo, Federico Scoto, Federico Turetta, Clara Zangrando, Roberta Barbante, Carlo Gambaro, Andrea 2021 text/tab-separated-values https://dx.doi.org/10.1594/pangaea.928097 https://doi.pangaea.de/10.1594/PANGAEA.928097 en eng PANGAEA - Data Publisher for Earth & Environmental Science https://dx.doi.org/10.1594/pangaea.928238 https://dx.doi.org/10.1016/j.chemosphere.2018.12.147 https://dx.doi.org/10.1016/j.scitotenv.2020.142954 https://dx.doi.org/10.1016/j.scitotenv.2019.136089 https://dx.doi.org/10.5194/acp-15-5457-2015 https://dx.doi.org/10.1016/j.atmosenv.2018.02.052 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 CC-BY aerosol amino acids Arctic ions organic acids sugars DATE/TIME Size fraction, upper threshold Size fraction, lower threshold Sample ID L-Alanine D-Alanine Glycine L-Arginine D-Arginine L-Threonine D-Threonine Serine Histidine Ornithine L-Aspartic acid D-Aspartic acid L-Glutamic acid D-Glutamic acid L-Leucine and Isoleucine D-Leucine and Isoleucine L-Phenylalanine D-Phenylalanine L-Proline L-Hydroxyproline D-Hydroxyproline L-Valine D-Valine Multiple investigations High Performance Liquid Chromatography tandem mass spectrometry HPLC-MS/MS Joint Arctic Weather Stations Dataset dataset 2021 ftdatacite https://doi.org/10.1594/pangaea.928097 https://doi.org/10.1594/pangaea.928238 https://doi.org/10.1016/j.chemosphere.2018.12.147 https://doi.org/10.1016/j.scitotenv.2020.142954 https://doi.org/10.1016/j.scitotenv.2019.136089 https://doi.org/10.51 2022-02-09T13:18:14Z Combined amino acids (CAA) were quantified in 7 aerosol samples collected during the Arctic campaign at Gruvebadet Laboratory in 2015. Combined amino acids (CAA) refer to amino acids contained in peptides, polipeptides and proteins.Aerosol samples were collected in quartz fiber filters. They were spiked with isotopically labelled standard solutions (2 - 3 µg/ml) prior extraction. CAA content was measured using HPLC-MS. : For the analysis of CAAs, in a 1.5 mL vial, 500 mL of each sample extract was mixed with 500 mL of 12M HCl and 5 mL of ascorbic acid at a concentration of 1 ng/mL to avoid oxidation of the amino acids. The vial was flushed with a stream of nitrogen before sealing and placed in a heat-block at 110 °C for 24 h with a Liebig prototype glass condenser used to condense compounds in the vapor (gas) phase back down to the liquid phase under boiling conditions through heat exchange with the environment. The hydrolysis products were freeze-dried to eliminate traces of HCl. The dry residues were subsequently dissolved in 500 mL of ultrapure water and diluted 1:10 before analysis for total amino acids (TAAs). The extracts of the field blank filters were also hydrolyzed so all TAAs concentrations could be blank corrected. The concentrations of the CAAs were calculated as the difference between the TAAs and free amino acids (FAAs) concentrations.Values below the detection limit of the measurements are indicated (<0.xxxxx). Dataset Arctic Svalbard DataCite Metadata Store (German National Library of Science and Technology) Arctic Svalbard |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
English |
topic |
aerosol amino acids Arctic ions organic acids sugars DATE/TIME Size fraction, upper threshold Size fraction, lower threshold Sample ID L-Alanine D-Alanine Glycine L-Arginine D-Arginine L-Threonine D-Threonine Serine Histidine Ornithine L-Aspartic acid D-Aspartic acid L-Glutamic acid D-Glutamic acid L-Leucine and Isoleucine D-Leucine and Isoleucine L-Phenylalanine D-Phenylalanine L-Proline L-Hydroxyproline D-Hydroxyproline L-Valine D-Valine Multiple investigations High Performance Liquid Chromatography tandem mass spectrometry HPLC-MS/MS Joint Arctic Weather Stations |
spellingShingle |
aerosol amino acids Arctic ions organic acids sugars DATE/TIME Size fraction, upper threshold Size fraction, lower threshold Sample ID L-Alanine D-Alanine Glycine L-Arginine D-Arginine L-Threonine D-Threonine Serine Histidine Ornithine L-Aspartic acid D-Aspartic acid L-Glutamic acid D-Glutamic acid L-Leucine and Isoleucine D-Leucine and Isoleucine L-Phenylalanine D-Phenylalanine L-Proline L-Hydroxyproline D-Hydroxyproline L-Valine D-Valine Multiple investigations High Performance Liquid Chromatography tandem mass spectrometry HPLC-MS/MS Joint Arctic Weather Stations Feltracco, Matteo Barbaro, Elena Spolaor, Andrea Vecchiato, Marco Callegaro, Alice Burgay, Francois Vardè, Massimiliano Maffezzoli, Niccolò Dallo, Federico Scoto, Federico Turetta, Clara Zangrando, Roberta Barbante, Carlo Gambaro, Andrea Combined amino acids in aerosol samples from Ny-Alesund, Svalbard Island, 2015 |
topic_facet |
aerosol amino acids Arctic ions organic acids sugars DATE/TIME Size fraction, upper threshold Size fraction, lower threshold Sample ID L-Alanine D-Alanine Glycine L-Arginine D-Arginine L-Threonine D-Threonine Serine Histidine Ornithine L-Aspartic acid D-Aspartic acid L-Glutamic acid D-Glutamic acid L-Leucine and Isoleucine D-Leucine and Isoleucine L-Phenylalanine D-Phenylalanine L-Proline L-Hydroxyproline D-Hydroxyproline L-Valine D-Valine Multiple investigations High Performance Liquid Chromatography tandem mass spectrometry HPLC-MS/MS Joint Arctic Weather Stations |
description |
Combined amino acids (CAA) were quantified in 7 aerosol samples collected during the Arctic campaign at Gruvebadet Laboratory in 2015. Combined amino acids (CAA) refer to amino acids contained in peptides, polipeptides and proteins.Aerosol samples were collected in quartz fiber filters. They were spiked with isotopically labelled standard solutions (2 - 3 µg/ml) prior extraction. CAA content was measured using HPLC-MS. : For the analysis of CAAs, in a 1.5 mL vial, 500 mL of each sample extract was mixed with 500 mL of 12M HCl and 5 mL of ascorbic acid at a concentration of 1 ng/mL to avoid oxidation of the amino acids. The vial was flushed with a stream of nitrogen before sealing and placed in a heat-block at 110 °C for 24 h with a Liebig prototype glass condenser used to condense compounds in the vapor (gas) phase back down to the liquid phase under boiling conditions through heat exchange with the environment. The hydrolysis products were freeze-dried to eliminate traces of HCl. The dry residues were subsequently dissolved in 500 mL of ultrapure water and diluted 1:10 before analysis for total amino acids (TAAs). The extracts of the field blank filters were also hydrolyzed so all TAAs concentrations could be blank corrected. The concentrations of the CAAs were calculated as the difference between the TAAs and free amino acids (FAAs) concentrations.Values below the detection limit of the measurements are indicated (<0.xxxxx). |
format |
Dataset |
author |
Feltracco, Matteo Barbaro, Elena Spolaor, Andrea Vecchiato, Marco Callegaro, Alice Burgay, Francois Vardè, Massimiliano Maffezzoli, Niccolò Dallo, Federico Scoto, Federico Turetta, Clara Zangrando, Roberta Barbante, Carlo Gambaro, Andrea |
author_facet |
Feltracco, Matteo Barbaro, Elena Spolaor, Andrea Vecchiato, Marco Callegaro, Alice Burgay, Francois Vardè, Massimiliano Maffezzoli, Niccolò Dallo, Federico Scoto, Federico Turetta, Clara Zangrando, Roberta Barbante, Carlo Gambaro, Andrea |
author_sort |
Feltracco, Matteo |
title |
Combined amino acids in aerosol samples from Ny-Alesund, Svalbard Island, 2015 |
title_short |
Combined amino acids in aerosol samples from Ny-Alesund, Svalbard Island, 2015 |
title_full |
Combined amino acids in aerosol samples from Ny-Alesund, Svalbard Island, 2015 |
title_fullStr |
Combined amino acids in aerosol samples from Ny-Alesund, Svalbard Island, 2015 |
title_full_unstemmed |
Combined amino acids in aerosol samples from Ny-Alesund, Svalbard Island, 2015 |
title_sort |
combined amino acids in aerosol samples from ny-alesund, svalbard island, 2015 |
publisher |
PANGAEA - Data Publisher for Earth & Environmental Science |
publishDate |
2021 |
url |
https://dx.doi.org/10.1594/pangaea.928097 https://doi.pangaea.de/10.1594/PANGAEA.928097 |
geographic |
Arctic Svalbard |
geographic_facet |
Arctic Svalbard |
genre |
Arctic Svalbard |
genre_facet |
Arctic Svalbard |
op_relation |
https://dx.doi.org/10.1594/pangaea.928238 https://dx.doi.org/10.1016/j.chemosphere.2018.12.147 https://dx.doi.org/10.1016/j.scitotenv.2020.142954 https://dx.doi.org/10.1016/j.scitotenv.2019.136089 https://dx.doi.org/10.5194/acp-15-5457-2015 https://dx.doi.org/10.1016/j.atmosenv.2018.02.052 |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1594/pangaea.928097 https://doi.org/10.1594/pangaea.928238 https://doi.org/10.1016/j.chemosphere.2018.12.147 https://doi.org/10.1016/j.scitotenv.2020.142954 https://doi.org/10.1016/j.scitotenv.2019.136089 https://doi.org/10.51 |
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