Seawater carbonate chemistry and sperm physiology in Mytilus galloprovincialis
The ongoing increase of CO2 in the atmosphere is inducing a progressive lowering of marine water pH that is predicted to decrease to 7.8 by the end of this century. In marine environment, physical perturbation may affect reproduction, which is crucial for species' survival and strictly depends...
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Language: | English |
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PANGAEA - Data Publisher for Earth & Environmental Science
2020
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Online Access: | https://dx.doi.org/10.1594/pangaea.925816 https://doi.pangaea.de/10.1594/PANGAEA.925816 |
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ftdatacite:10.1594/pangaea.925816 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
English |
topic |
Animalia Benthic animals Benthos Bottles or small containers/Aquaria <20 L Coast and continental shelf Field experiment Laboratory experiment Mediterranean Sea Mollusca Mytilus galloprovincialis Other studied parameter or process Reproduction FOS Medical biotechnology Single species Temperate Type Species Registration number of species Uniform resource locator/link to reference Experiment Treatment Experiment day Sperm concentration Sperm concentration, standard deviation Sperm motility Sperm motility, standard deviation Viability Viability, standard deviation Mitochondrial membrane potential Mitochondrial membrane potential, standard deviation pH, intracellular pH, intracellular, standard deviation pH, seminal pH, seminal, standard deviation Reactive oxygen species production Reactive oxygen species production, standard deviation Lipid peroxidation Lipid peroxidation, standard deviation Temperature, water Temperature, water, standard deviation Salinity Salinity, standard deviation pH pH, standard deviation Alkalinity, total Alkalinity, total, standard deviation Partial pressure of carbon dioxide water at sea surface temperature wet air Partial pressure of carbon dioxide, standard deviation Carbon, inorganic, dissolved Carbon, inorganic, dissolved, standard deviation Carbonate system computation flag Carbon dioxide Carbon dioxide, standard deviation Fugacity of carbon dioxide water at sea surface temperature wet air Fugacity of carbon dioxide in seawater, standard deviation Bicarbonate ion Bicarbonate ion, standard deviation Carbonate ion Carbonate ion, standard deviation Aragonite saturation state Aragonite saturation state, standard deviation Calcite saturation state Calcite saturation state, standard deviation Potentiometric Potentiometric titration Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. 2010 Calculated using seacarb after Orr et al. 2018 Ocean Acidification International Coordination Centre OA-ICC |
spellingShingle |
Animalia Benthic animals Benthos Bottles or small containers/Aquaria <20 L Coast and continental shelf Field experiment Laboratory experiment Mediterranean Sea Mollusca Mytilus galloprovincialis Other studied parameter or process Reproduction FOS Medical biotechnology Single species Temperate Type Species Registration number of species Uniform resource locator/link to reference Experiment Treatment Experiment day Sperm concentration Sperm concentration, standard deviation Sperm motility Sperm motility, standard deviation Viability Viability, standard deviation Mitochondrial membrane potential Mitochondrial membrane potential, standard deviation pH, intracellular pH, intracellular, standard deviation pH, seminal pH, seminal, standard deviation Reactive oxygen species production Reactive oxygen species production, standard deviation Lipid peroxidation Lipid peroxidation, standard deviation Temperature, water Temperature, water, standard deviation Salinity Salinity, standard deviation pH pH, standard deviation Alkalinity, total Alkalinity, total, standard deviation Partial pressure of carbon dioxide water at sea surface temperature wet air Partial pressure of carbon dioxide, standard deviation Carbon, inorganic, dissolved Carbon, inorganic, dissolved, standard deviation Carbonate system computation flag Carbon dioxide Carbon dioxide, standard deviation Fugacity of carbon dioxide water at sea surface temperature wet air Fugacity of carbon dioxide in seawater, standard deviation Bicarbonate ion Bicarbonate ion, standard deviation Carbonate ion Carbonate ion, standard deviation Aragonite saturation state Aragonite saturation state, standard deviation Calcite saturation state Calcite saturation state, standard deviation Potentiometric Potentiometric titration Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. 2010 Calculated using seacarb after Orr et al. 2018 Ocean Acidification International Coordination Centre OA-ICC Gallo, Alessandra Esposito, Maria Consiglia Cuccaro, Alessia Buia, Maria-Cristina Tarallo, Andrea Monfrecola, Vincenzo Tosti, Elisabetta Boni, Raffaele Seawater carbonate chemistry and sperm physiology in Mytilus galloprovincialis |
topic_facet |
Animalia Benthic animals Benthos Bottles or small containers/Aquaria <20 L Coast and continental shelf Field experiment Laboratory experiment Mediterranean Sea Mollusca Mytilus galloprovincialis Other studied parameter or process Reproduction FOS Medical biotechnology Single species Temperate Type Species Registration number of species Uniform resource locator/link to reference Experiment Treatment Experiment day Sperm concentration Sperm concentration, standard deviation Sperm motility Sperm motility, standard deviation Viability Viability, standard deviation Mitochondrial membrane potential Mitochondrial membrane potential, standard deviation pH, intracellular pH, intracellular, standard deviation pH, seminal pH, seminal, standard deviation Reactive oxygen species production Reactive oxygen species production, standard deviation Lipid peroxidation Lipid peroxidation, standard deviation Temperature, water Temperature, water, standard deviation Salinity Salinity, standard deviation pH pH, standard deviation Alkalinity, total Alkalinity, total, standard deviation Partial pressure of carbon dioxide water at sea surface temperature wet air Partial pressure of carbon dioxide, standard deviation Carbon, inorganic, dissolved Carbon, inorganic, dissolved, standard deviation Carbonate system computation flag Carbon dioxide Carbon dioxide, standard deviation Fugacity of carbon dioxide water at sea surface temperature wet air Fugacity of carbon dioxide in seawater, standard deviation Bicarbonate ion Bicarbonate ion, standard deviation Carbonate ion Carbonate ion, standard deviation Aragonite saturation state Aragonite saturation state, standard deviation Calcite saturation state Calcite saturation state, standard deviation Potentiometric Potentiometric titration Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. 2010 Calculated using seacarb after Orr et al. 2018 Ocean Acidification International Coordination Centre OA-ICC |
description |
The ongoing increase of CO2 in the atmosphere is inducing a progressive lowering of marine water pH that is predicted to decrease to 7.8 by the end of this century. In marine environment, physical perturbation may affect reproduction, which is crucial for species' survival and strictly depends on gamete quality. The effects of seawater acidification (SWAc) on gamete quality of broadcast spawning marine invertebrates result largely from experiments of gamete exposure while the SWAc impact in response to adult exposure is poorly investigated. Performing microcosm and in field experiments at a naturally acidified site, we investigated the effects of adult SWAc exposure on sperm quality parameters underlying fertilization in Mytilus galloprovincialis. These animals were exposed to pH 7.8 over 21 days and collected at different times to analyze sperm parameters as concentration, motility, viability, morphology, oxidative status, intra- and extra-cellular pH and mitochondrial membrane potential. Results obtained in the two experimental approaches were slightly different. Under field conditions, we found an increase in total sperm motility and mitochondrial membrane potential on days 7 and 14 from the start of SWAc exposure whereas, in microcosm, SWAc group showed an increase of total motility on day 14. In addition, sperm morphology and intracellular pH were affected in both experimental approaches; whereas oxidative stress was detected only in spermatozoa collected from mussels under natural SWAc. The overall analysis suggests that, in mussels, SWAc toxic mechanism in spermatozoa does not involve oxidative stress. This study represents the first report on mussel sperm quality impairment after adult SWAc exposure, which may affect fertilization success with negative ecological and economic consequences; it also indicates that, although naturally acidified areas represent ideal natural laboratories for investigating the impact of ocean acidification, microcosm experiments are necessary for examining action mechanisms. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2020) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-12-11. |
format |
Dataset |
author |
Gallo, Alessandra Esposito, Maria Consiglia Cuccaro, Alessia Buia, Maria-Cristina Tarallo, Andrea Monfrecola, Vincenzo Tosti, Elisabetta Boni, Raffaele |
author_facet |
Gallo, Alessandra Esposito, Maria Consiglia Cuccaro, Alessia Buia, Maria-Cristina Tarallo, Andrea Monfrecola, Vincenzo Tosti, Elisabetta Boni, Raffaele |
author_sort |
Gallo, Alessandra |
title |
Seawater carbonate chemistry and sperm physiology in Mytilus galloprovincialis |
title_short |
Seawater carbonate chemistry and sperm physiology in Mytilus galloprovincialis |
title_full |
Seawater carbonate chemistry and sperm physiology in Mytilus galloprovincialis |
title_fullStr |
Seawater carbonate chemistry and sperm physiology in Mytilus galloprovincialis |
title_full_unstemmed |
Seawater carbonate chemistry and sperm physiology in Mytilus galloprovincialis |
title_sort |
seawater carbonate chemistry and sperm physiology in mytilus galloprovincialis |
publisher |
PANGAEA - Data Publisher for Earth & Environmental Science |
publishDate |
2020 |
url |
https://dx.doi.org/10.1594/pangaea.925816 https://doi.pangaea.de/10.1594/PANGAEA.925816 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
https://CRAN.R-project.org/package=seacarb https://dx.doi.org/10.1016/j.envpol.2020.115063 https://CRAN.R-project.org/package=seacarb |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1594/pangaea.925816 https://doi.org/10.1016/j.envpol.2020.115063 |
_version_ |
1766158286597390336 |
spelling |
ftdatacite:10.1594/pangaea.925816 2023-05-15T17:51:12+02:00 Seawater carbonate chemistry and sperm physiology in Mytilus galloprovincialis Gallo, Alessandra Esposito, Maria Consiglia Cuccaro, Alessia Buia, Maria-Cristina Tarallo, Andrea Monfrecola, Vincenzo Tosti, Elisabetta Boni, Raffaele 2020 text/tab-separated-values https://dx.doi.org/10.1594/pangaea.925816 https://doi.pangaea.de/10.1594/PANGAEA.925816 en eng PANGAEA - Data Publisher for Earth & Environmental Science https://CRAN.R-project.org/package=seacarb https://dx.doi.org/10.1016/j.envpol.2020.115063 https://CRAN.R-project.org/package=seacarb Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 CC-BY Animalia Benthic animals Benthos Bottles or small containers/Aquaria <20 L Coast and continental shelf Field experiment Laboratory experiment Mediterranean Sea Mollusca Mytilus galloprovincialis Other studied parameter or process Reproduction FOS Medical biotechnology Single species Temperate Type Species Registration number of species Uniform resource locator/link to reference Experiment Treatment Experiment day Sperm concentration Sperm concentration, standard deviation Sperm motility Sperm motility, standard deviation Viability Viability, standard deviation Mitochondrial membrane potential Mitochondrial membrane potential, standard deviation pH, intracellular pH, intracellular, standard deviation pH, seminal pH, seminal, standard deviation Reactive oxygen species production Reactive oxygen species production, standard deviation Lipid peroxidation Lipid peroxidation, standard deviation Temperature, water Temperature, water, standard deviation Salinity Salinity, standard deviation pH pH, standard deviation Alkalinity, total Alkalinity, total, standard deviation Partial pressure of carbon dioxide water at sea surface temperature wet air Partial pressure of carbon dioxide, standard deviation Carbon, inorganic, dissolved Carbon, inorganic, dissolved, standard deviation Carbonate system computation flag Carbon dioxide Carbon dioxide, standard deviation Fugacity of carbon dioxide water at sea surface temperature wet air Fugacity of carbon dioxide in seawater, standard deviation Bicarbonate ion Bicarbonate ion, standard deviation Carbonate ion Carbonate ion, standard deviation Aragonite saturation state Aragonite saturation state, standard deviation Calcite saturation state Calcite saturation state, standard deviation Potentiometric Potentiometric titration Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. 2010 Calculated using seacarb after Orr et al. 2018 Ocean Acidification International Coordination Centre OA-ICC dataset Dataset 2020 ftdatacite https://doi.org/10.1594/pangaea.925816 https://doi.org/10.1016/j.envpol.2020.115063 2021-11-05T12:55:41Z The ongoing increase of CO2 in the atmosphere is inducing a progressive lowering of marine water pH that is predicted to decrease to 7.8 by the end of this century. In marine environment, physical perturbation may affect reproduction, which is crucial for species' survival and strictly depends on gamete quality. The effects of seawater acidification (SWAc) on gamete quality of broadcast spawning marine invertebrates result largely from experiments of gamete exposure while the SWAc impact in response to adult exposure is poorly investigated. Performing microcosm and in field experiments at a naturally acidified site, we investigated the effects of adult SWAc exposure on sperm quality parameters underlying fertilization in Mytilus galloprovincialis. These animals were exposed to pH 7.8 over 21 days and collected at different times to analyze sperm parameters as concentration, motility, viability, morphology, oxidative status, intra- and extra-cellular pH and mitochondrial membrane potential. Results obtained in the two experimental approaches were slightly different. Under field conditions, we found an increase in total sperm motility and mitochondrial membrane potential on days 7 and 14 from the start of SWAc exposure whereas, in microcosm, SWAc group showed an increase of total motility on day 14. In addition, sperm morphology and intracellular pH were affected in both experimental approaches; whereas oxidative stress was detected only in spermatozoa collected from mussels under natural SWAc. The overall analysis suggests that, in mussels, SWAc toxic mechanism in spermatozoa does not involve oxidative stress. This study represents the first report on mussel sperm quality impairment after adult SWAc exposure, which may affect fertilization success with negative ecological and economic consequences; it also indicates that, although naturally acidified areas represent ideal natural laboratories for investigating the impact of ocean acidification, microcosm experiments are necessary for examining action mechanisms. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2020) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-12-11. Dataset Ocean acidification DataCite Metadata Store (German National Library of Science and Technology) |