Seawater carbonate chemistry and gene expression in the gills of juvenile turbot (Psetta maxima)

• Main Authors: Hermann, Bernd Timo, Würtz, Sven, Vanselow, Klaus Heinrich, Schulz, Carsten, Stiller, Kevin Torben
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2019
• Subjects: Animalia; Chordata; Coast and continental shelf; Containers and aquaria 20-1000 L or < 1 m**2; Gene expression incl. proteomics; Laboratory experiment; Nekton; North Atlantic; Other studied parameter or process; Pelagos; Psetta maxima; Single species; Temperate; Type; Species; Registration number of species; Uniform resource locator/link to reference; Experiment duration; Sample type; Sample ID; Treatment; Partial pressure of carbon dioxide water at sea surface temperature wet air; Replicate; CNRQs, Quantitative reverse transcription PCR; Gill, hypertrophied chloride cells; Gill, hyperplasia on basis of primary filament; Gill, not affected; Gill, hypertrophy of secondary lamellae; Gill, hyperplasia on distal part of secondary lamellae; Gill, general hyperplasia; Gill, lamellar clubbing; Salinity; Temperature, water; Partial pressure of carbon dioxide, standard deviation; pH; pH, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Carbon, inorganic, dissolved; Aragonite saturation state; Calcite saturation state; Quantitative reverse transcription polymerase chain reaction qRT-PCR; Microscopy; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification; Turbot
• Online Access: https://dx.doi.org/10.1594/pangaea.920153; https://doi.pangaea.de/10.1594/PANGAEA.920153

The data sets stem from a study which aimed to investigate the impact of chronic severe hypercapnia on the cellular stress response in the gills of juvenile turbot (Psetta maxima). The experiment was conducted in the so called respirometer, a recirculating aquaculture respirometer system (RARS) which allowed the persistent, stable manipulation of carbon dioxide concentrations. Juvenile turbot (Psetta maxima) where subjected to different levels of environmental hypercapnia (low: ~3000 μatm, medium: ~15000 μatm, high: ~25000 μatm). After eight weeks, fish where sacrificed and gill samples were taken for further histological and transcriptional analysis. For histological investigation of the gills, samples where fixed in 4% phosphate-buffered formalin and examined via a microscope equipped with a digital camera. In order to investigate changes in the transcriptome, a fraction of the gill samples was stored in RNAlater. Relative gene expression analysis was conducted via RT-qPCR, using a Fluidigm chip system. Calibrated normalized relative quantities (CNRQs) where assessed with the QBASE software. This included the evaluation of the most suitable combination of reference genes with the help of the GeNorm algorithm. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2019) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-07-07.