Seawater carbonate chemistry and physiology and functional gene expression of the coccolithophore Emiliania huxleyi ...

• Main Authors: Feng, Yuanyuan, Roleda, Michael Y, Armstrong, Evelyn, Summerfield, Tina C, Law, Cliff S, Hurd, Catriona L, Boyd, Philip W
• Format: Dataset
• Language: English
• Published: PANGAEA 2020
• Subjects: Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria <20 L; Calcification/Dissolution; Chromista; Emiliania huxleyi; Gene expression incl. proteomics; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Light; Macro-nutrients; Not applicable; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Temperature; Type; Species; Registration number of species; Uniform resource locator/link to reference; Bottle number; Treatment; Growth rate; Net photosynthesis rate, per cell; Calcification rate of carbon per cell; Ratio; Carbon, organic, particulate, per cell; Carbon, inorganic, particulate, per cell; Nitrogen, organic, particulate, per cell; Phosphorus, organic, particulate, per cell; Chlorophyll a per cell; Gene expression, fold change, relative; Gene expression, fold change, relative, standard deviation; Salinity; Temperature, water; Alkalinity, total; Carbon, inorganic, dissolved; pH; Fugacity of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Carbon dioxide; Calcite saturation state; Carbonate system computation flag; Aragonite saturation state; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.919895; https://doi.pangaea.de/10.1594/PANGAEA.919895

Here, we examined two-way and multiple driver effects of ocean acidification and other key environmental drivers - nitrate, phosphate, irradiance, and temperature - on the growth, photosynthetic and calcification rates, and the elemental composition of E. huxleyi. In addition, changes in functional gene expression were examined to understand the molecular mechanisms underpinning the physiological responses. The single driver manipulation experiments sugguest decreased nitrate supply being the most important driver regulating E. huxleyi physiology, by significantly reducing the growth, photosynthetic and calcification rates. In addition, the interaction of ocean acidification and decreased nitrate supply (projected for year 2100) had more negative synergistic effects on E. huxleyi physiology than all other two-way factorial manipulations, suggesting a linkage between the single dominant driver (nitrate) effects and interactive effects with other drivers. Simultaneous manipulation of all five environmental ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2019) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-07-07. ...