Seawater carbonate chemistry and swimming performance of Tritia reticulata (L.) veligers

• Main Authors: Fonseca, Joana G, Laranjeiro, Filipe, Freitas, Daniela B, Oliveira, Isabel B, Rocha, Rui J M, Hinzmann, Mariana, Barroso, Carlos M, Galante-Oliveira, Susana
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2020
• Subjects: Animalia; Behaviour; Bottles or small containers/Aquaria <20 L; Coast and continental shelf; Development; Laboratory experiment; Mollusca; Mortality/Survival; North Atlantic; Pelagos; Single species; Temperate; Temperature; Tritia reticulata; Zooplankton; Type; Species; Registration number of species; Uniform resource locator/link to reference; Treatment; Day of experiment; Mortality; Stage; Larval stages; Larvae; Activity; Activity, standard error; Distance; Distance, standard error; Angle; Percentage; Salinity; Temperature, water; pH; Alkalinity, total; Partial pressure of carbon dioxide water at sea surface temperature wet air; Carbonate ion; Calcite saturation state; Aragonite saturation state; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbon, inorganic, dissolved; Experiment; Potentiometric; Potentiometric titration; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.919252; https://doi.pangaea.de/10.1594/PANGAEA.919252

Tritia reticulata (L.) is a neogastropod ubiquitous in the coastal communities of the NE Atlantic. Its life cycle relies on the swimming performance of planktonic early life stages, whose sensitivity to the climate conditions projected for the near future, namely of ocean acidification (OA) and warming (W), is, to our best knowledge, unknown. To examine the resilience of larval stages to future environmental conditions, this work investigates the effect of OA-W on the swimming performance of T. reticulata veligers under a range of experimental conditions, based on the end-of-century projections of the Intergovernmental Panel on Climate Change. Veligers were exposed to six experimental scenarios for 14 days, employing a full factorial design with three temperatures (T°C: 18, 20 and 22 °C) and two pH levels (pHtarget: 8.1 and 7.8). Mortality was assessed throughout the trial, after which swimming behaviour – characterised by the activity, speed and the distance travelled by veligers – was analysed by automated video recordings in a Zebrabox device. Mortality increased with OA-W and, although more active, larvae travelled shorter distances revealing reduced swimming speed under acidic and warmer conditions, with the interaction of the tested stressors – pH and T°C – being highly significant. Results motivated the morpho-histological analysis of larvae preserved at the end of the trial, to check for the integrity of the organs involved in veligers' motion: statocysts, velum and foot. Statocyst and velar morpho-structure were conserved but histological damage of metapodial epithelia was evident under acidity, namely an apparent hypertrophy and protrusion of the secretory cells, with dispersed pigmented granules and, at 22 °C, less cilia, with potential functional implications. Negative consequences of the OA-W scenarios tested on veligers' competence are unveiled, pointing towards the eminent threat these phenomena constitute to T. reticulata perpetuation in case no mitigation measures are taken, and projections become effective. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2019) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-06-12.