Seawater carbonate chemistry and photosynthetic pigments and photophysiology of polar microalga Chlorella sp.

• Main Authors: Tan, Yong Hao, Lim, Phaik Eem, Beardall, John, Poong, Sze Wan, Phang, Siew Moi
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2019
• Subjects: Bottles or small containers/Aquaria <20 L; Chlorella sp.; Chlorophyta; Growth/Morphology; Laboratory experiment; Laboratory strains; Not applicable; Phytoplankton; Plantae; Primary production/Photosynthesis; Single species; Type; Species; Day of experiment; Treatment; Cell density; Cell density, standard deviation; Growth rate; Growth rate, standard deviation; Chlorophyll a per cell; Chlorophyll a, standard deviation; Carotenoids per cell; Carotenoids, standard deviation; Ratio; Ratio, standard deviation; Diameter; Diameter, standard deviation; Surface area; Surface area, standard deviation; Volume; Cell biovolume, standard deviation; Cell surface area/cell volume ratio; Cell surface area/cell volume, standard deviation; Photochemical quantum yield; Photochemical quantum yield, standard deviation; Maximum light utilization efficiency; Maximum light utilization efficiency, standard deviation; Maximal electron transport rate, relative; Maximal electron transport rate, relative, standard deviation; Non photochemical quenching, maximum; Non photochemical quenching, standard deviation; Light saturation; Light saturation, standard deviation; Temperature, water; Salinity; Salinity, standard deviation; pH; pH, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Bicarbonate ion; Bicarbonate ion, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Carbonate ion; Aragonite saturation state; Calcite saturation state; Potentiometric; Potentiometric titration; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Antarctic; Antarc*; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.916160; https://doi.pangaea.de/10.1594/PANGAEA.916160

Ocean acidification, due to increased levels of anthropogenic carbon dioxide, is known to affect the physiology and growth of marine phytoplankton, especially in polar regions. However, the effect of acidification or carbonation on cellular metabolism in polar marine phytoplankton still remains an open question. There is some evidence that small chlorophytes may benefit more than other taxa of phytoplankton. To understand further how green polar picoplankton could acclimate to high oceanic CO2, studies were conducted on an Antarctic Chlorella sp. Chlorella sp. maintained its growth rate (∼0.180 /day), photosynthetic quantum yield (Fv/Fm = ∼0.69) and chlorophyll a (0.145 fg/cell) and carotenoid (0.06 fg/cell) contents under high CO2, while maximum rates of electron transport decreased and non-photochemical quenching increased under elevated CO2. GCMS-based metabolomic analysis reveal that this polar Chlorella strain modulated the levels of metabolites associated with energy, amino acid, fatty acid and carbohydrate production, which could favour its survival in an increasingly acidified ocean. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2019) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-05-6.