Seawater carbonate chemistry and biochemical composition of the coccolithophore Emiliania huxleyi

• Main Authors: Heidenreich, Elena, Wördenweber, Robin, Kirschhöfer, Frank, Nusser, Michael, Friedrich, Frank, Fahl, Kirsten, Kruse, Olaf, Rost, Björn, Franzreb, Matthias, Brenner-Weiß, Gerald, Rokitta, Sebastian D
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2019
• Subjects: Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria <20 L; Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Light; Not applicable; Pelagos; Phytoplankton; Single species; Type; Species; Registration number of species; Uniform resource locator/link to reference; Treatment; Alkenones; Alkenones, standard deviation; Temperature, water; Temperature, water, standard deviation; Growth rate; Growth rate, standard deviation; Cell, diameter; Cell, diameter, standard deviation; Name; Category; Concentration; Concentration, standard deviation; Salinity; pH; pH, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Bicarbonate ion; Bicarbonate ion, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Aragonite saturation state; Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.913444; https://doi.pangaea.de/10.1594/PANGAEA.913444

Owing to the hierarchical organization of biology, from genomes over transcriptomes and proteomes down to metabolomes, there is continuous debate about the extent to which data and interpretations derived from one level, e.g. the transcriptome, are in agreement with other levels, e.g. the metabolome. Here, we tested the effect of ocean acidification (OA; 400 vs. 1000 μatm CO2) and its modulation by light intensity (50 vs. 300 μmol photons m-2 s-1) on the biomass composition (represented by 75 key metabolites) of diploid and haploid life-cycle stages of the coccolithophore Emiliania huxleyi (RCC1216 and RCC1217) and compared these data with interpretations from previous physiological and gene expression screenings. The metabolite patterns showed minor responses to OA in both life-cycle stages. Whereas previous gene expression analyses suggested that the observed increased biomass buildup derived from lipid and carbohydrate storage, this dataset suggests that OA slightly increases overall biomass of cells, but does not significantly alter their metabolite composition. Generally, light was shown to be a more dominant driver of metabolite composition than OA, increasing the relative abundances of amino acids, mannitol and storage lipids, and shifting pigment contents to accommodate increased irradiance levels. The diploid stage was shown to contain vastly more osmolytes and mannitol than the haploid stage, which in turn had a higher relative content of amino acids, especially aromatic ones. Besides the differences between the investigated cell types and the general effects on biomass buildup, our analyses indicate that OA imposes only negligible effects on E. huxleyi's biomass composition. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2019) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-03-06.