Seawater carbonate chemistry and metabolism of bacterial communities from coastal waters

• Main Authors: Fuentes-Lema, Antonio, Sanleón-Bartolomé, Henar, Lubian, L M, Sobrino, Cristina
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2018
• Subjects: Biomass/Abundance/Elemental composition; Coast and continental shelf; Containers and aquaria 20-1000 L or < 1 m**2; Entire community; Laboratory experiment; North Atlantic; Other; Other metabolic rates; Pelagos; Respiration; Temperate; Type; Treatment; Experiment duration; Chlorophyll a; Chlorophyll a, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Carbon, organic, dissolved; Carbon, organic, dissolved, standard deviation; pH; pH, standard deviation; Respiration rate, oxygen; Respiration rate, oxygen, standard deviation; Respiration rate, oxygen, cumulative; Respiration rate, oxygen, cumulative, standard deviation; Bacteria; Bacteria, standard deviation; Bacterial production; Bacterial production, standard deviation; Bacteria, carbon demand; Bacteria, carbon demand, standard deviation; Bacteria, carbon demand, cumulative; Bacteria, carbon demand, cumulative, standard deviation; Bacteria, growth efficiency; Bacteria, growth efficiency, standard deviation; Temperature, water; Temperature, water, standard deviation; Salinity; Salinity, standard deviation; Carbon dioxide; Carbon dioxide, standard deviation; Bicarbonate ion; Bicarbonate ion, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Carbonate system computation flag; Fugacity of carbon dioxide water at sea surface temperature wet air; Fugacity of carbon dioxide in seawater, standard deviation; Aragonite saturation state; Aragonite saturation state, standard deviation; Calcite saturation state; Calcite saturation state, standard deviation; Experiment; Calculated using seacarb after Nisumaa et al. 2010; Calculated using seacarb after Orr et al. 2018; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.912775; https://doi.pangaea.de/10.1594/PANGAEA.912775

Microcosm experiments to assess the bacterioplankton's response to phytoplankton-derived organic matter obtained under current and future ocean CO2 levels were performed. Surface seawater enriched with inorganic nutrients was bubbled for 8 days with air (current CO2 scenario) or with a 1000ppm CO2 air mixture (future CO2 scenario) under solar radiation. The organic matter produced under the current and future CO2 scenarios was subsequently used as an inoculum. Triplicate 12L flasks filled with 1.2µm of filtered natural seawater enriched with the organic matter inocula were incubated in the dark for 8 days under CO2 conditions simulating current and future CO2 scenarios, to study the bacterial response. The acidification of the media increased bacterial respiration at the beginning of the experiment, while the addition of the organic matter produced under future levels of CO2 was related to changes in bacterial production and abundance. This resulted in a 67% increase in the integrated bacterial respiration under future CO2 conditions compared to present CO2 conditions and 41% higher integrated bacterial abundance with the addition of the acidified organic matter compared to samples with the addition of non acidified organic matter. This study demonstrates that the increase in atmospheric CO2 levels can impact bacterioplankton metabolism directly, by changes in the respiration rate, and indirectly, by changes on the organic matter, which affected bacterial production and abundance. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2019) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-02-28.