Seawater carbonate chemistry and body size, change in allele frequency of Strongylocentrotus purpuratus larvae ...

• Main Authors: Brennan, Reid S, Garrett, April D, Huber, Kaitlin E, Hargarten, Heidi, Pespeni, Melissa H
• Format: Dataset
• Language: English
• Published: PANGAEA 2019
• Subjects: Animalia; Bottles or small containers/Aquaria <20 L; Coast and continental shelf; Echinodermata; Gene expression incl. proteomics; Laboratory experiment; North Pacific; Pelagos; Single species; Strongylocentrotus purpuratus; Temperate; Zooplankton; Type; Species; Registration number of species; Uniform resource locator/link to reference; Treatment; Identification; Body size; Name; Base pair size; Probability; Change; Temperature, water; Temperature, water, standard deviation; Salinity; Salinity, standard deviation; pH; pH, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Carbon, inorganic, dissolved; Aragonite saturation state; Calcite saturation state; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.912484; https://doi.pangaea.de/10.1594/PANGAEA.912484

Standing genetic variation is important for population persistence in extreme environmental conditions. While some species may have the capacity to adapt to predicted average future global change conditions, the ability to survive extreme events is largely unknown. We used single-generation selection experiments on hundreds of thousands of Strongylocentrotus purpuratus sea urchin larvae generated from wild-caught adults to identify adaptive genetic variation responsive to moderate (pH 8.0) and extreme (pH 7.5) low-pH conditions. Sequencing genomic DNA from pools of larvae, we identified consistent changes in allele frequencies across replicate cultures for each pH condition and observed increased linkage disequilibrium around selected loci, revealing selection on recombined standing genetic variation. We found that loci responding uniquely to either selection regime were at low starting allele frequencies while variants that responded to both pH conditions (11.6% of selected variants) started at high ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2019) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-02-17. ...