Seawater carbonate chemistry and mussel shell defense capacity of Mytilus coruscus, supplement to: Zhao, Xinguo; Han, Yu; Chen, Bijuan; Xia, Bin; Qu, Keming; Liu, Guangxu (2020): CO2-driven ocean acidification weakens mussel shell defense capacity and induces global molecular compensatory responses. Chemosphere, 243, 125415

• Main Authors: Zhao, Xinguo, Han, Yu, Chen, Bijuan, Xia, Bin, Qu, Keming, Liu, Guangxu
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2020
• Subjects: Acid-base regulation; Animalia; Benthic animals; Benthos; Bottles or small containers/Aquaria <20 L; Coast and continental shelf; Growth/Morphology; Laboratory experiment; Mollusca; Mytilus coruscus; North Pacific; Other studied parameter or process; Single species; Temperate; Type; Species; Registration number of species; Uniform resource locator/link to reference; Experiment duration; Treatment; Replicate; Area; Percentage; Shell strength; Size; Haemolymph, pH; Haemolymph, calcium ion; pH; pH, standard deviation; Temperature, water; Temperature, water, standard deviation; Salinity; Salinity, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Aragonite saturation state; Aragonite saturation state, standard deviation; Calcite saturation state; Calcite saturation state, standard deviation; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Experiment; Potentiometric; Potentiometric titration; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Pacific; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.911746; https://doi.pangaea.de/10.1594/PANGAEA.911746

Oceanic uptake of atmospheric CO2 is reducing seawater pH and shifting carbonate chemistry within, a process termed as ocean acidification (OA). Marine mussels are a family of ecologically and economically significant bivalves that are widely distributed along coastal areas worldwide. Studies have demonstrated that OA greatly disrupts mussels' physiological functions. However, the underlying molecular responses (e.g., whether there were any molecular compensation mechanisms) and the extent to which OA affects mussel shell defense capacity remain largely unknown. In this study, the thick shell mussels Mytilus coruscus were exposed to the ambient pH (8.1) or one of two lowered pH levels (7.8 and 7.4) for 40 days. The results suggest that future OA will damage shell structure and weaken shell strength and shell closure strength, ultimately reducing mussel shell defense capacity. In addition, future OA will also disrupt haemolymph pH and Ca2+ homeostasis, leading to extracellular acidosis and Ca2+ deficiency. Mantle transcriptome analyses indicate that mussels will adopt a series of molecular compensatory responses to mitigate these adverse effects; nevertheless, weakened shell defense capacity will increase mussels' susceptibility to predators, parasites and pathogens, and thereby reduce their fitness. Overall, the findings of this study have significant ecological and economic implications, and will enhance our understanding of the future of the mussel aquaculture industry and coastal ecosystems. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2019) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-02-03.