Seawater carbonate chemistry and hatching, survival and development rate of Calanus pacificus, supplement to: McLaskey, Anna K; McElhany, Paul; Busch, Shallin D; Maher, Michael; Winans, Amanda K; Keister, Julie E (2019): Early life stages of Calanus pacificus are neither exposed nor sensitive to low pH waters. Journal of Plankton Research, fbz059

• Main Authors: McLaskey, Anna K, McElhany, Paul, Busch, Shallin D, Maher, Michael, Winans, Amanda K, Keister, Julie E
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2019
• Subjects: Animalia; Arthropoda; Bottles or small containers/Aquaria <20 L; Calanus pacificus; Coast and continental shelf; Laboratory experiment; Mortality/Survival; North Pacific; Pelagos; Reproduction; FOS Medical biotechnology; Single species; Temperate; Zooplankton; Type; Species; Registration number of species; Uniform resource locator/link to reference; Experiment; Treatment; Identification; Hatching rate; Hatching rate, standard deviation; Proportion of survival; Proportion of survival, standard deviation; Proportion; Proportion, standard deviation; Temperature, water; pH; pH, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Salinity; Salinity, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Carbon, inorganic, dissolved; Aragonite saturation state; Calcite saturation state; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Maher; Pacific; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.910338; https://doi.pangaea.de/10.1594/PANGAEA.910338

We characterized the vertical distribution of Calanus pacificus eggs and larvae and the carbonate chemistry that they are exposed to in Puget Sound, WA. We found that, under stratified conditions, more than 90% of eggs and nauplii stages 1–4 were distributed above the pycnocline, in seawater with pH higher than 7.7. In addition, eggs and larvae from 101 females were reared for 5 days under a range of pH conditions (7.2–8.0) to investigate how pH sensitivity varies among individuals. We observed a slight increase in naupliar survival at pH 7.3 in Individual Brood experiments, while in Mixed Brood experiments, exposure to pH 7.3 led to a small decline in hatching success. In a Split Brood experiment, inter-individual variability among different females' broods masked pH effects. These results indicate that C. pacificus early life stages are generally tolerant to short-term direct effects of ocean acidification. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2019) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2019-12-20.