Seawater carbonate chemistry and carbon, oxygen and boron isotopes within Ochrosphaera neapolitana, supplement to: Liu, Yiwei; Eagle, Robert A; Aciego, Sarah M; Gilmore, Rosaleen E; Ries, Justin B (2018): A coastal coccolithophore maintains pH homeostasis and switches carbon sources in response to ocean acidification. Nature Communications, 9(1)

• Main Authors: Liu, Yiwei, Eagle, Robert A, Aciego, Sarah M, Gilmore, Rosaleen E, Ries, Justin B
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2018
• Subjects: Bottles or small containers/Aquaria <20 L; Chromista; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Ochrosphaera neapolitana; Other studied parameter or process; Pelagos; Phytoplankton; Single species; Type; Species; Registration number of species; Uniform resource locator/link to reference; Identification; Temperature, water; Temperature, water, standard deviation; Salinity; Salinity, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; pH; pH, standard deviation; Bicarbonate ion; Bicarbonate ion, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbon dioxide; Carbon dioxide, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; δ13C, dissolved inorganic carbon; δ11B; δ11B, standard deviation; δ13C, particulate inorganic carbon; Difference; Difference, standard deviation; δ18O; δ13C, particulate organic carbon; Carbon, organic, particulate; Carbon, organic, particulate, standard deviation; Carbon; Carbon, total, standard deviation; Carbon, inorganic, particulate; Particulate inorganic carbon/particulate organic carbon ratio; Carbonate system computation flag; Fugacity of carbon dioxide water at sea surface temperature wet air; Fugacity of carbon dioxide in seawater, standard deviation; Aragonite saturation state; Aragonite saturation state, standard deviation; Calcite saturation state; Calcite saturation state, standard deviation; Potentiometric titration; Potentiometric; Calculated using CO2SYS; Mass spectrometry; Calculated using seacarb after Nisumaa et al. 2010; Calculated using seacarb after Orr et al. 2018; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.909063; https://doi.pangaea.de/10.1594/PANGAEA.909063

Ocean acidification will potentially inhibit calcification by marine organisms; however, the response of the most prolific ocean calcifiers, coccolithophores, to this perturbation remains under characterized. Here we report novel chemical constraints on the response of the widespread coccolithophore species Ochrosphaera neapolitana (O. neapolitana) to changing-CO2 conditions. We cultured this algae under three pCO2-controlled seawater pH conditions (8.05, 8.22, and 8.33). Boron isotopes within the algae's extracellular calcite plates show that this species maintains a constant pH at the calcification site, regardless of CO2-induced changes in pH of the surrounding seawater. Carbon and oxygen isotopes in the algae's calcite plates and carbon isotopes in the algae's organic matter suggest that O. neapolitana utilize carbon from a single internal dissolved inorganic carbon (DIC) pool for both calcification and photosynthesis, and that a greater proportion of dissolved CO2 relative to HCO3− enters the internal DIC pool under acidified conditions. These two observations may explain how O. neapolitana continues calcifying and photosynthesizing at a constant rate under different atmospheric-pCO2 conditions. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2019) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2019-11-20.