Seawater carbonate chemistry and gastric pH homeostasis and larval recruitment in the sea star Asterias rubens, supplement to: Hu, Marian Y; Lein, E; Bleich, Markus; Melzner, Frank; Stumpp, Meike (2018): Trans-life cycle acclimation to experimental ocean acidification affects gastric pH homeostasis and larval recruitment in the sea star Asterias rubens. Acta Physiologica, 224(2), e13075

• Main Authors: Hu, Marian Y, Lein, E, Bleich, Markus, Melzner, Frank, Stumpp, Meike
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2018
• Subjects: Acid-base regulation; Animalia; Asterias rubens; Baltic Sea; Behaviour; Benthic animals; Benthos; Bottles or small containers/Aquaria <20 L; Coast and continental shelf; Echinodermata; Growth/Morphology; Laboratory experiment; Reproduction; FOS Medical biotechnology; Single species; Temperate; Zooplankton; Type; Species; Registration number of species; Uniform resource locator/link to reference; Treatment; Growth rate; Growth rate, standard deviation; Feeding rate per individual; Feeding rate, standard deviation; Feeding rate, standard error; Fecundity; Fecundity, standard deviation; Fecundity, standard error; Change; Change, standard deviation; Change, standard error; Duration, number of days; Identification; Larvae; Length; Length, standard deviation; Length, standard error; Larvae, standard deviation; Larvae, standard error; pH; pH, standard deviation; pH, standard error; Proton gradients; Proton gradients, standard error; Ouabain; Ethylisopropyl amiloride; Respiration rate, oxygen, per individual; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Temperature, water; Temperature, water, standard deviation; Salinity; Salinity, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Calcite saturation state; Calcite saturation state, standard deviation; Aragonite saturation state; Aragonite saturation state, standard deviation; Carbonate system computation flag; Carbon dioxide; Carbon dioxide, standard deviation; Fugacity of carbon dioxide water at sea surface temperature wet air; Fugacity of carbon dioxide in seawater, standard deviation; Bicarbonate ion; Bicarbonate ion, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Experiment; Calculated using seacarb after Nisumaa et al. 2010; Calculated using seacarb after Orr et al. 2018; Ocean Acidification International Coordination Centre OA-ICC; Marian; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.907313; https://doi.pangaea.de/10.1594/PANGAEA.907313

Aim: Experimental simulation of near‐future ocean acidification (OA) has been demonstrated to affect growth and development of echinoderm larval stages through energy allocation towards ion and pH compensatory processes. To date, it remains largely unknown how major pH regulatory systems and their energetics are affected by trans‐generational exposure to near‐future acidification levels.Methods: Here, we used the common sea star Asterias rubens in a reciprocal transplant experiment comprising different combinations of OA scenarios, to study trans‐generational plasticity using morphological and physiological endpoints.Results: Acclimation of adults to pHT 7.2 (pCO2 3500 μatm) led to reductions in feeding rates, gonad weight and fecundity. No effects were evident at moderate acidification levels (pHT 7.4; pCO2 2000 μatm). Parental pre‐acclimation to pHT 7.2 for 85 days reduced developmental rates even when larvae were raised under moderate and high pH conditions, whereas pre‐acclimation to pHT 7.4 did not alter offspring performance. Microelectrode measurements and pharmacological inhibitor studies carried out on larval stages demonstrated that maintenance of alkaline gastric pH represents a substantial energy sink under acidified conditions that may contribute up to 30% to the total energy budget.Conclusion: Parental pre‐acclimation to acidification levels that are beyond the pH that is encountered by this population in its natural habitat (eg, pHT 7.2) negatively affected larval size and development, potentially through reduced energy transfer. Maintenance of alkaline gastric pH and reductions in maternal energy reserves probably constitute the main factors for a reduced juvenile recruitment of this marine keystone species under simulated OA. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2019) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2019-09-30.