Seawater carbonate chemistry and survival, swimming activity, feeding rate of Northern shrimp (Pandalus borealis), supplement to: Bechmann, Renée Katrin; Lyng, Emily; Westerlund, Stig; Bamber, Shaw D; Berry, Mark; Arnberg, Maj; Kringstad, Alfhild; Calosi, Piero; Seear, Paul J (2018): Early life stages of Northern shrimp (Pandalus borealis) are sensitive to fish feed containing the anti-parasitic drug diflubenzuron. Aquatic Toxicology, 198, 82-91

• Main Authors: Bechmann, Renée Katrin, Lyng, Emily, Westerlund, Stig, Bamber, Shaw D, Berry, Mark, Arnberg, Maj, Kringstad, Alfhild, Calosi, Piero, Seear, Paul J
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2018
• Subjects: Animalia; Arthropoda; Behaviour; Bottles or small containers/Aquaria <20 L; Coast and continental shelf; Gene expression incl. proteomics; Laboratory experiment; Mortality/Survival; North Atlantic; Other; Pandalus borealis; Pelagos; Single species; Temperate; Temperature; Zooplankton; Type; Species; Registration number of species; Uniform resource locator/link to reference; Experiment; Treatment; Time in days; Percentage; Percentage, standard deviation; Number; Stage; Swimming activity, beam breaks per hour; Swimming activity, standard deviation; Individuals; Feeding rate per individual; Gene name; Gene expression, fold change, relative; Gene expression, fold change, relative, standard error; Salinity; Temperature, water; pH; Alkalinity, total; Carbon, inorganic, dissolved; Partial pressure of carbon dioxide water at sea surface temperature wet air; Calcite saturation state; Aragonite saturation state; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Kringstad; northern shrimp; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.903546; https://doi.pangaea.de/10.1594/PANGAEA.903546

Increasing use of fish feed containing the chitin synthesis inhibiting anti-parasitic drug diflubenzuron (DFB) in salmon aquaculture has raised concerns over its impact on coastal ecosystems. Larvae of Northern shrimp (Pandalus borealis) were exposed to DFB medicated feed under Control conditions (7.0 °C, pH 8.0) and under Ocean Acidification and Warming conditions (OAW, 9.5 °C and pH 7.6). Two weeks' exposure to DFB medicated feed caused significantly increased mortality. The effect of OAW and DFB on mortality of shrimp larvae was additive; 10% mortality in Control, 35% in OAW, 66% in DFB and 92% in OAW + DFB. In OAW + DFB feeding and swimming activity were reduced for stage II larvae and none of the surviving larvae developed to stage IV. Two genes involved in feeding (GAPDH and PRLP) and one gene involved in moulting (DD9B) were significantly downregulated in larvae exposed to OAW + DFB relative to the Control. Due to a shorter intermoult period under OAW conditions, the OAW + DFB larvae were exposed throughout two instead of one critical pre-moult period. This may explain the more serious sub-lethal effects for OAW + DFB than DFB larvae. A single day exposure at 4 days after hatching did not affect DFB larvae, but high mortality was observed for OAW + DFB larvae, possibly because they were exposed closer to moulting. High mortality of shrimp larvae exposed to DFB medicated feed, indicates that the use of DFB in salmon aquaculture is a threat to crustacean zooplankton. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2019-07-01.