Seawater carbonate chemistry and growth, physiological performance of the Manila clam Ruditapes philippinarum, supplement to: Zhao, Liqiang; Yang, Feng; Milano, Stefania; Han, Tiankun; Walliser, Eric Otto; Schöne, Bernd R (2018): Transgenerational acclimation to seawater acidification in the Manila clam Ruditapes philippinarum: Preferential uptake of metabolic carbon. Science of the Total Environment, 627, 95-103

• Main Authors: Zhao, Liqiang, Yang, Feng, Milano, Stefania, Han, Tiankun, Walliser, Eric Otto, Schöne, Bernd R
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2018
• Subjects: Animalia; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria 20-1000 L or < 1 m**2; Growth/Morphology; Laboratory experiment; Mollusca; North Pacific; Other studied parameter or process; Respiration; Ruditapes philippinarum; Single species; Temperate; Type; Species; Registration number of species; Uniform resource locator/link to reference; Treatment; Growth rate; Growth rate, standard deviation; Condition index; Condition index, standard deviation; Metabolic rate of oxygen; Metabolic rate of oxygen, standard deviation; δ13C; δ13C, standard deviation; Percentage; Temperature, water; Temperature, water, standard deviation; Salinity; Salinity, standard deviation; pH; pH, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Bicarbonate ion; Bicarbonate ion, standard deviation; Carbonate ion; Carbonate ion, standard deviation; δ13C, dissolved inorganic carbon; δ13C, dissolved inorganic carbon, standard deviation; Carbonate system computation flag; Carbon dioxide; Carbon dioxide, standard deviation; Fugacity of carbon dioxide water at sea surface temperature wet air; Fugacity of carbon dioxide in seawater, standard deviation; Aragonite saturation state; Aragonite saturation state, standard deviation; Calcite saturation state; Calcite saturation state, standard deviation; Experiment; Calculated using seacarb after Nisumaa et al. 2010; Calculated using seacarb after Orr et al. 2018; Ocean Acidification International Coordination Centre OA-ICC; Pacific; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.902985; https://doi.pangaea.de/10.1594/PANGAEA.902985

Ocean acidification may interfere with the calcifying physiology of marine bivalves. Therefore, understanding their capacity for acclimation and adaption to low pH over multiple generations is crucial to make predictions about the fate of this economically and ecologically important fauna in an acidifying ocean. Transgenerational exposure to an acidification scenario projected by the end of the century (i.e., pH 7.7) has been shown to confer resilience to juvenile offspring of the Manila clam, Ruditapes philippinarum. However, whether, and to what extent, this resilience can persist into adulthood are unknown and the mechanisms driving transgenerational acclimation remain poorly understood. The present study takes observations of Manila clam juveniles further into the adult stage and observes similar transgenerational responses. Under acidified conditions, clams originating from parents reproductively exposed to the same level of low pH show a significantly faster shell growth rate, a higher condition index and a lower standard metabolic rate than those without prior history of transgenerational acclimation. Further analyses of stable carbon isotopic signatures in dissolved inorganic carbon of seawater, individual soft tissues and shells reveal that up to 61% of shell carbonate comes from metabolic carbon, suggesting that transgenerationally acclimated clams may preferentially extract internal metabolic carbon rather than transport external seawater inorganic carbon to build shells, the latter known to be energetically expensive. While a large metabolic carbon contribution (45%) is seen in non-acclimated clams, a significant reduction in the rate of shell growth indicates it might occur at the expense of other calcification-relevant processes. It therefore seems plausible that, following transgenerational acclimation, R. philippinarum can implement a less costly and more efficient energy-utilizing strategy to mitigate the impact of seawater acidification. Collectively, our findings indicate that marine bivalves are more resilient to ocean acidification projected for the end of the century than previously thought. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2019-06-26.