Seawater carbonate chemistry and the microbiome of the octocoral Lobophytum pauciflorum, supplement to: Wessels, Wiebke; Sprungala, Susanne; Watson, Sue-Ann; Miller, David J; Bourne, David G (2017): The microbiome of the octocoral Lobophytum pauciflorum: minor differences between sexes and resilience to short-term stress. FEMS Microbiology Ecology, 93(5)

• Main Authors: Wessels, Wiebke, Sprungala, Susanne, Watson, Sue-Ann, Miller, David J, Bourne, David G
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2017
• Subjects: Animalia; Benthic animals; Benthos; Cnidaria; Coast and continental shelf; Community composition and diversity; Containers and aquaria 20-1000 L or < 1 m**2; Laboratory experiment; Lobophytum pauciflorum; Single species; South Pacific; Temperature; Tropical; Type; Species; Registration number of species; Uniform resource locator/link to reference; Experiment duration; Experiment; Treatment; Operational taxonomic unit; Operational taxonomic unit, standard deviation; Alpha diversity; Alpha diversity, standard deviation; Shannon Diversity Index; Shannon Diversity Index, standard deviation; Simpson's index; Simpson's index, standard deviation; Class; Family; Sequence abundance; Sequence abundance, standard error; Salinity; Salinity, standard deviation; Temperature, water; Temperature, water, standard deviation; pH; pH, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Carbon, inorganic, dissolved; Aragonite saturation state; Calcite saturation state; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Pacific; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.893397; https://doi.pangaea.de/10.1594/PANGAEA.893397

Bacteria associated with marine invertebrates are thought to have a range of important roles that benefit the host including production of compounds that may exclude pathogenic microorganisms and recycling of essential nutrients. This study characterised the microbiome of a gonochoric octocoral, Lobophytum pauciflorum, and investigated whether either sex or environmental stresses influenced the diversity of the associated microbiome through amplicon profiling of the bacterial 16S rRNA gene. Sequences affiliated to Spirochaetaceae and Endozoicimonaceae dominated the microbiome of L. pauciflorum, representing 43% and 21% of the community, respectively. Among the dominant class affiliations, no sex-specific differences were detected, though unassigned sequences were at a 2-fold higher relative abundance in samples from female individuals than from males. These potentially novel sequences contributed to observed differences between sexes as detected by a multivariate analysis at the OTU level. Exposing L. pauciflorum fragments to increased temperature (31°C), decreased pH (7.9) or both stressors simultaneously for 12 days did not significantly alter the microbial community, indicating that the soft coral microbiome is relatively resilient to short-term environmental stress. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2018-09-04.