Seawater carbonate chemistry and cytoskeleton, lysozymes, and nitric oxide in hemocytes of blood clams, Tegillarca granosa. ...

• Main Authors: Su, Wenhao, Rong, Jiahuan, Zha, Shanjie, Yan, Maocang, Fang, Jun, Liu, Guangxu
• Format: Dataset
• Language: English
• Published: PANGAEA 2018
• Subjects: Animalia; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria 20-1000 L or < 1 m**2; Gene expression incl. proteomics; Laboratory experiment; Mollusca; North Pacific; Other studied parameter or process; Single species; Tegillarca granosa; Temperate; Type; Species; Registration number of species; Uniform resource locator/link to reference; Experiment duration; pH, NBS scale; Replicate; Phagocytosis rate; Fluorescence; Nitric oxide in hemocytes, per cell; Lysozyme in hemocytes, per cell; Lysozyme enzyme activity, per cell; Gene name; Gene expression, fold change, relative; Gene expression, fold change, relative, standard deviation; Temperature, water; Temperature, water, standard error; Salinity; Salinity, standard error; pH, standard error; Alkalinity, total; Alkalinity, total, standard error; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide water at sea surface temperature wet air, standard error; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard error; Aragonite saturation state; Aragonite saturation state, standard error; Calcite saturation state; Calcite saturation state, standard error; Carbonate system computation flag; pH, total scale; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Experiment; Pacific; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.892892; https://doi.pangaea.de/10.1594/PANGAEA.892892

An enormous amount of anthropogenic carbon dioxide (CO2) has been dissolved into the ocean, leading to a lower pH and changes in the chemical properties of seawater, which has been termed ocean acidification (OA). The impacts of pCO2-driven acidification on immunity have been revealed recently in various marine organisms. However, the mechanism causing the reduction in phagocytosis still remains unclear. Therefore, the impacts of pCO2-driven OA at present and near-future levels (pH values of 8.1, 7.8, and 7.4) on the rate of phagocytosis, the abundance of cytoskeleton components, the levels of nitric oxide (NO), and the concentration and activity of lysozymes (LZM) of hemocytes were investigated in a commercial bivalve species, the blood clam (Tegillarca granosa). In addition, the effects of OA on the expression of genes regulating actin skeleton and nitric oxide synthesis 2 (NOS2) were also analyzed. The results obtained showed that the phagocytic rate, cytoskeleton component abundance, concentration and ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2018-08-06. ...