Effects of exposure to different target pCO2 levels on development and biochemical responses related with oxidative stress and energy metabolism during the crustacean Homarus gammarus (L.) larval development, supplement to: Rato, Lénia D; Novais, Sara C; Lemos, Marco F L; Alves, Luís M F; Leandro, Sérgio M (2017): Homarus gammarus (Crustacea: Decapoda) larvae under an ocean acidification scenario: responses across different levels of biological organization. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 203, 29-38

• Main Authors: Rato, Lénia D, Novais, Sara C, Lemos, Marco F L, Alves, Luís M F, Leandro, Sérgio M
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2017
• Subjects: Animalia; Arthropoda; Coast and continental shelf; Containers and aquaria 20-1000 L or < 1 m**2; Development; Growth/Morphology; Homarus gammarus; Laboratory experiment; Mortality/Survival; North Atlantic; Other metabolic rates; Pelagos; Single species; Temperate; Zooplankton; Type; Species; Registration number of species; Uniform resource locator/link to reference; Treatment; Life stage; Superoxide dismutase activity, unit per protein mass; Lactate dehydrogenase activity, per protein mass; Electron transport system activity, per wet mass; Lipid peroxidation, per wet mass; DNA damage, per protein; Carbohydrate; Wet mass; Carapace, length; Time in days; Status; Temperature, water; Temperature, water, standard deviation; Salinity; Salinity, standard deviation; pH; pH, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Bicarbonate ion; Bicarbonate ion, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Oxygen; Oxygen, standard deviation; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Carbon, inorganic, dissolved; Aragonite saturation state; Calcite saturation state; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.892416; https://doi.pangaea.de/10.1594/PANGAEA.892416

The present study evaluated the effects of exposure to different target pCO2 levels: control (C: 370μatm, pH = 8.15) and ocean acidification (OA: 710μatm, pH = 7.85) on development and biochemical responses related with oxidative stress and energy metabolism during the crustacean Homarus gammarus (L.) larval development, integrating different levels of biological organization. After hatching in the laboratory, larvae from the same female brood were exposed to the described conditions from hatching until reaching Stage III (last larval stage – 11 days). H. gammarus larvae demonstrated some susceptibility when addressing the predicted pCO2 levels for 2100. Further analysis at the biochemical and physiological level highlighted the occurrence of oxidative stress in the OA scenario (Superoxide Dismutase reduction and higher DNA damage) that was followed by developmental effects, increased inter-moult period from SII to SIII and reduced growth. The extended exposure to these conditions may affect organisms' key life-cycle functions such as physiological resistance, growth, sexual maturation, or reproduction with implications in their future fitness and population dynamics. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2018-07-12.