Seawater carbonate chemistry,total mercury and methylmercury accumulation, catalase, superoxide dismutase and glutathione-S-transferase activities of a commercially-important fish (Argyrosomus regius), supplement to: Sampaio, Eduardo; Lopes, Ana R; Francisco, Sofia; Paula, José Ricardo; Pimentel, Marta; Maulvault, Ana L; Repolho, Tiago; Grilo, Tiago F; Pousão-Ferreira, Pedro; Marques, António; Rosa, Rui (2018): Ocean acidification dampens physiological stress response to warming and contamination in a commercially-important fish ( Argyrosomus regius ). Science of the Total Environment, 618, 388-398

• Main Authors: Sampaio, Eduardo, Lopes, Ana R, Francisco, Sofia, Paula, José Ricardo, Pimentel, Marta, Maulvault, Ana L, Repolho, Tiago, West, Adam G, Pousão-Ferreira, Pedro, Marques, António, Rosa, Rui
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2018
• Subjects: Animalia; Argyrosomus regius; Chordata; Containers and aquaria 20-1000 L or < 1 m**2; Inorganic toxins; Laboratory experiment; Laboratory strains; Nekton; North Atlantic; Other metabolic rates; Other studied parameter or process; Pelagos; Single species; Temperature; Type; Species; Registration number of species; Uniform resource locator/link to reference; Experiment duration; Temperature, water; pH; Treatment; Identification; Fulton's condition factor; Tissues; Mercury; Malondialdehyde, per protein mass; Glutathione S-transferase, activity per protein mass; Catalase activity, per protein mass; Superoxide dismutase activity, per protein mass; Heat shock protein, per protein mass; Temperature, water, standard deviation; Salinity; Salinity, standard deviation; pH, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Aragonite saturation state; Aragonite saturation state, standard deviation; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Ferreira; Ricardo; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.892277; https://doi.pangaea.de/10.1594/PANGAEA.892277

Increases in carbon dioxide (CO2) and other greenhouse gases emissions are changing ocean temperature and carbonate chemistry (warming and acidification, respectively). Moreover, the simultaneous occurrence of highly toxic and persistent contaminants, such as methylmercury, will play a key role in further shaping the ecophysiology of marine organisms. Despite recent studies reporting mostly additive interactions between contaminant and climate change effects, the consequences of multi-stressor exposure are still largely unknown. Here we disentangled how Argyrosomus regius physiology will be affected by future stressors, by analysing organ-dependent mercury (Hg) accumulation (gills, liver and muscle) within isolated/combined warming (delta T = 4 °C) and acidification (ΔpCO2 = 1100 μatm) scenarios, as well as direct deleterious effects and phenotypic stress response over multi-stressor contexts. After 30 days of exposure, although no mortalities were observed in any treatments, Hg concentration was enhanced under warming conditions, especially in the liver. On the other hand, elevated CO2 decreased Hg accumulation and consistently elicited a dampening effect on warming and contamination-elicited oxidative stress (catalase, superoxide dismutase and glutathione-S-transferase activities) and heat shock responses. Thus, potentially unpinned on CO2-promoted protein removal and ionic equilibrium between hydrogen and reactive oxygen species, we found that co-occurring acidification decreased heavy metal accumulation and contributed to physiological homeostasis. Although this indicates that fish can be physiologically capable of withstanding future ocean conditions, additional experiments are needed to fully understand the biochemical repercussions of interactive stressors (additive, synergistic or antagonistic). : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2018-07-02.