Seawater carbonate chemistry and photosynthetic performance of Thalassiosira (Conticribra) weissflogii (Bacillariophyta), supplement to: Yang, Yuling; Li, Wei; Li, Zhenzhen; Xu, Juntian (2018): Combined effects of ocean acidification and nutrient levels on the photosynthetic performance of Thalassiosira (Conticribra) weissflogii (Bacillariophyta). Phycologia, 57(2), 121-129

• Main Authors: Yang, Yuling, Li, Wei, Li, Zhenzhen, Xu, Juntian
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2018
• Subjects: Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria <20 L; Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; Macro-nutrients; Not applicable; Ochrophyta; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Thalassiosira weissflogii; Type; Species; Registration number of species; Uniform resource locator/link to reference; Figure; Treatment; Growth rate; Growth rate, standard deviation; Chlorophyll a per cell; Chlorophyll a, standard deviation; Carotenoids per cell; Carotenoids, standard deviation; Carotenoids/Chlorophyll a ratio; Carotenoids/Chlorophyll a ratio, standard deviation; Maximum photochemical quantum yield of photosystem II; Maximum photochemical quantum yield of photosystem II, standard deviation; Effective quantum yield; Effective quantum yield, standard deviation; Non photochemical quenching; Non photochemical quenching, standard deviation; Irradiance; Electron transport rate, relative; Electron transport rate, relative, standard deviation; Maximal electron transport rate, relative; Photosynthetic efficiency; Photosynthetic efficiency, standard deviation; Light saturation point; Light saturation point, standard deviation; Salinity; Temperature, water; Silicate; Nitrate; Phosphate; pH; pH, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Bicarbonate ion; Bicarbonate ion, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbon dioxide; Carbon dioxide, standard deviation; Carbonate system computation flag; Fugacity of carbon dioxide water at sea surface temperature wet air; Aragonite saturation state; Calcite saturation state; Potentiometric; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.888942; https://doi.pangaea.de/10.1594/PANGAEA.888942

The purpose of this study was to investigate the effects of ocean acidification and nutrient level on the growth and photosynthetic performance of the diatom Thalassiosira (Conticribra) weissflogii. Cells were exposed to varying levels of CO2 [current CO2 (LC), 400 μatm; high CO2 (HC), 1000 μatm] and nutrients, with NO3− and PO43− concentrations enriched, respectively, at 50 μmol/l and 5 μmol/l [high nutrient (HN)], 20 μmol/l and 2 μmol/l [mid-level nutrient (MN)] and 10 μmol/l and 1 μmol/l [low nutrient (LN)]. After acclimatization for over 20 generations, no significant differences in growth rates were observed between LC and HC cultures under both HN and LN conditions; whereas, HC significantly reduced the growth rate under MN conditions. Lower nutrient loading significantly inhibited the growth rates of both LC and HC cultures; whereas, HC (but not LC) significantly decreased chlorophyll a and carotenoid contents in LN treatments. HC conditions significantly increased maximum relative electron transport rates (rETRmax) and saturating light intensity (Ik) of HN cultures, with rETRmax showing a positive relationship with growth rates stimulated by nutrient enrichments. The maximum (Fv/Fm) and effective quantum yield (Yield) were all inhibited under LN conditions, with the greatest reduction in Yield observed under LC conditions, corresponding to the highest nonphotochemical quenching, lowest light use efficiency (α) and lowest rETRmax. Based on these results, ocean acidification and nutrient availability may influence photosynthetic performance in T. weissflogii individually or interactively, with the future growth of marine diatoms mediated by these codependent environmental drivers. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2018-04-11.