Seawater carbonate chemistry and sperm-egg collisions, gamete fusion, and generation of Ca2+ oscillations of a broadcast spawning bivalve, Tegillarca granosa ...

• Main Authors: Shi, Wei, Han, Yu, Guo, Cheng, Zhao, Xinguo, Liu, Saixi, Su, Wenhao, Wang, Yichen, Zha, Shanjie, Chai, Xueliang, Liu, Guangxu
• Format: Dataset
• Language: English
• Published: PANGAEA 2017
• Subjects: Animalia; Benthic animals; Benthos; Bottles or small containers/Aquaria <20 L; Coast and continental shelf; Laboratory experiment; Mollusca; North Pacific; Other metabolic rates; Reproduction; FOS Medical biotechnology; Single species; Tegillarca granosa; Temperate; Type; Species; Registration number of species; Uniform resource locator/link to reference; Treatment; Replicate; Fertilization success rate; pH; pH, standard error; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide water at sea surface temperature wet air, standard error; Salinity; Salinity, standard error; Temperature, water; Temperature, water, standard error; Alkalinity, total; Alkalinity, total, standard error; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard error; Aragonite saturation state; Aragonite saturation state, standard error; Calcite saturation state; Calcite saturation state, standard error; Probability; Fluorescence intensity; Amplitude; Curvilinear velocity; Curvilinear velocity, standard error; Average path velocity; Average path velocity, standard error; Straight line velocity; Straight line velocity, standard error; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Pacific; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.888184; https://doi.pangaea.de/10.1594/PANGAEA.888184

Although the effect of ocean acidification on fertilization success of marine organisms is increasingly well documented, the underlying mechanisms are not completely understood. The fertilization success of broadcast spawning invertebrates depends on successful sperm-egg collisions, gamete fusion, and standard generation of Ca2+oscillations. Therefore, the realistic effects of future ocean pCO2 levels on these specific aspects of fertilization of Tegillarca granosa were investigated in the present study through sperm velocity trials, fertilization kinetics model analysis, and intracellular Ca2+assays, respectively. Results obtained indicated that ocean acidification significantly reduced the fertilization success of T. granosa, which could be accountable by (i) decreased sperm velocity hence reducing the probability for sperm-egg collisions; (ii) lowered probability of gamete fusion for each gamete collision event; and (iii) disrupted intracellular Ca2+ oscillations. ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2018-04-09. ...