Bacterial and eukaryotic operational taxonomic units (OTU) in sea ice, water and deep-sea sediment samples of the Central Arctic collected during POLARSTERN cruise ARK-XXVII/3 (IceArc) in 2012 ...

• Main Authors: Rapp, Josephine Z, Fernández-Méndez, Mar, Bienhold, Christina, Boetius, Antje
• Format: Dataset
• Language: English
• Published: PANGAEA 2017
• Subjects: File content; File name; File format; File size; Uniform resource locator/link to file; Assessment of bacterial life and matter cycling in deep-sea surface sediments ABYSS; Arctic Ocean; Sea ice
• Online Access: https://dx.doi.org/10.1594/pangaea.882580; https://doi.pangaea.de/10.1594/PANGAEA.882580

We aimed to explore the community composition and turnover of eukaryotic and bacterial microorganisms associated with ice-associated and sinking algal aggregates, as well as their similarity to potential source communities of sea ice, water and deep-sea sediments using Illumina tag sequencing. We sampled algae aggregates growing in melt ponds on sea ice, deposited algae aggregates at the seafloor in more than 4000 m water depth, sea ice, upper water column, sediment surface and the gut content of holothurians feeding on the deposits. For Illumina sequencing, the Amplicon libraries of the bacterial V4-V6 region of the 16S rRNA gene and the eukaryotic V4 region of the 18S rRNA gene were generated according to the protocol recommended by Illumina (16S Metagenomic Sequencing Library Preparation, Part # 15044223, Rev. B). For Bacteria we selected the S-D-Bact-0564-a-S-15 and S-*Univ-1100-a-A-15 primer pair based on a primer evaluation by Klindworth et al. (2013, doi:10.1093/nar/gks808) and for Eukaryota the ... : Supplement to: Rapp, Josephine Z; Fernández-Méndez, Mar; Bienhold, Christina; Boetius, Antje (2018): Effects of ice-algal aggregate export on the connectivity of bacterial core communities in the central Arctic Ocean. Frontiers in Microbiology, 9 ...