Intact polar diacylglycerol (IP-DAG) lipids measured in cultures of four Antarctic diatom isolates ...

• Main Authors: Collins, James R, Ducklow, Hugh W, Marchetti, Adrian, Van Mooy, Benjamin A S
• Format: Dataset
• Language: English
• Published: PANGAEA 2017
• Subjects: Species; Strain; Sulfoquinovosyldiacylglycerol 368; Phosphatidylglycerol 325; Monogalactosyldiacylglycerol 328; Sulfoquinovosyldiacylglycerol 304; Digalactosyldiacylglycerol 327; Phosphatidylglycerol 304; Phosphatidylglycerol 347; Phosphatidylglycerol 3810; Sulfoquinovosyldiacylglycerol 347; Monogalactosyldiacylglycerol 369; Sulfoquinovosyldiacylglycerol 366; Digalactosyldiacylglycerol 325; Digalactosyldiacylglycerol 368; Sulfoquinovosyldiacylglycerol 334; Phosphatidylglycerol 367; Monogalactosyldiacylglycerol 3810; Sulfoquinovosyldiacylglycerol 447; Phosphatidylcholine 320; Phosphatidylcholine 4010; Sulfoquinovosyldiacylglycerol 365; Phosphatidylglycerol 301; Sulfoquinovosyldiacylglycerol 290; Phosphatidylethanolamine 409; Phosphatidylcholine 387; Monogalactosyldiacylglycerol 4211; Phosphatidylglycerol 300; Phosphatidylcholine 323; Monogalactosyldiacylglycerol 388; Sulfoquinovosyldiacylglycerol 342; Monogalactosyldiacylglycerol 409; Digalactosyldiacylglycerol 365; Monogalactosyldiacylglycerol 446; Phosphatidylcholine 389; Phosphatidylcholine 346; Phosphatidylcholine 369; Phosphatidylglycerol 345; Phosphatidylcholine 325; Sulfoquinovosyldiacylglycerol 323; Sulfoquinovosyldiacylglycerol 344; Sulfoquinovosyldiacylglycerol 387; Sulfoquinovosyldiacylglycerol 345; Sulfoquinovosyldiacylglycerol 302; Phosphatidylglycerol 4211; Sulfoquinovosyldiacylglycerol 388; Phosphatidylcholine 362; Monogalactosyldiacylglycerol 324; Sulfoquinovosyldiacylglycerol 321; Phosphatidylglycerol 322; Antarc*; Antarctic; Bellingshausen Sea
• Online Access: https://dx.doi.org/10.1594/pangaea.879617; https://doi.pangaea.de/10.1594/PANGAEA.879617

Diatoms were isolated by Adrian Marchetti (http://orcid.org/0000-0003-4608-4775) from waters of the Bellingshausen Sea and grown on replete media. Cells were collected onto 0.7 µm GF/F filters. Extraction was performed using a modified Bligh and Dyer (Bligh and Dyer, 1959; https://doi.org/10.1139/o59-099) method described in Popendorf et al. (2013; https://doi.org/10.1007/s11745-012-3748-0). Lipid extracts were analyzed by HPLC-ESI-MS with data dependent-MS2 acquisition on a high-resolution, accurate mass Thermo Q Exactive Hybrid Quadrupole-Orbitrap mass spectrometer (ThermoFisher Scientific, Waltham, MA, USA) coupled to an Agilent 1200 HPLC system (Agilent, Santa Clara, CA, USA). The HPLC-ESI-MS method is described in Collins et al., 2016 (https://doi.org/10.1021/acs.analchem.6b01260). The LOBSTAHS lipidomics discovery software (Collins et al., 2016; https://doi.org/10.1021/acs.analchem.6b01260) was used to putatively identify HPLC-MS features in the data. We confirmed each LOBSTAHS identification using two ...