Seawater carbonate chemistry and leaf coloration, photophysiology and photosynthetic pigments of seagrass Zostera noltii, supplement to: Repolho, Tiago; Duarte, Bernardo; Dionísio, Gisela; Paula, José Ricardo; Lopes, Ana R; Rosa, Inês C; Grilo, Tiago F; Cacador, Isabel; Calado, Ricardo; Rosa, Rui (2017): Seagrass ecophysiological performance under ocean warming and acidification. Scientific Reports, 7, 41443

• Main Authors: Repolho, Tiago, Duarte, Bernardo, Dionísio, Gisela, Paula, José Ricardo, Lopes, Ana R, Rosa, Inês C, Grilo, Tiago F, Cacador, Isabel, Calado, Ricardo, Rosa, Rui
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2017
• Subjects: Benthos; Bottles or small containers/Aquaria <20 L; Coast and continental shelf; Laboratory experiment; North Atlantic; Plantae; Primary production/Photosynthesis; Seagrass; Single species; Temperate; Tracheophyta; Zostera noltii; Type; Species; Registration number of species; Uniform resource locator/link to reference; Experiment duration; Temperature, water; pH; Shoots; Maximum photochemical quantum yield of photosystem II; Electron transport rate; Chlorophyll a; Chlorophyll b; Pheophytin a; Pheophytin b; Auroxanthin; Antheraxanthin; beta-Carotene; Lutein; Violaxanthin; Zeaxanthin; Chlorophyll total, per mass; Carotenoids; Ratio; De-epoxidation state; Temperature, water, standard deviation; Salinity; Salinity, standard deviation; pH, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Aragonite saturation state; Aragonite saturation state, standard deviation; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Calcite saturation state; Experiment; Potentiometric; Potentiometric titration; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Duarte; Ricardo; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.875791; https://doi.pangaea.de/10.1594/PANGAEA.875791

Seagrasses play an essential ecological role within coastal habitats and their worldwide population decline has been linked to different types of anthropogenic forces. We investigated, for the first time, the combined effects of future ocean warming and acidification on fundamental biological processes of Zostera noltii, including shoot density, leaf coloration, photophysiology (electron transport rate, ETR; maximum PSII quantum yield, Fv/Fm) and photosynthetic pigments. Shoot density was severely affected under warming conditions, with a concomitant increase in the frequency of brownish colored leaves (seagrass die-off). Warming was responsible for a significant decrease in ETR and Fv/Fm (particularly under control pH conditions), while promoting the highest ETR variability (among experimental treatments). Warming also elicited a significant increase in pheophytin and carotenoid levels, alongside an increase in carotenoid/chlorophyll ratio and De-Epoxidation State (DES). Acidification significantly affected photosynthetic pigments content (antheraxanthin, beta-carotene, violaxanthin and zeaxanthin), with a significant decrease being recorded under the warming scenario. No significant interaction between ocean acidification and warming was observed. Our findings suggest that future ocean warming will be a foremost determinant stressor influencing Z. noltii survival and physiological performance. Additionally, acidification conditions to occur in the future will be unable to counteract deleterious effects posed by ocean warming. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2017-05-26.