Seawater carbonate chemistry and germling growth rate, and male and female gametophyte sizes of kelps Macrocystis pyrifera and Undaria pinnatifida in laboratory experiment, supplement to: Leal, Pablo P; Hurd, Catriona L; Fernández, Pamela A; Roleda, Michael Y (2016): Meiospore development of the kelps Macrocystis pyrifera and Undaria pinnatifida under ocean acidification and ocean warming: independent effects are more important than their interaction. Marine Biology, 164(1)

• Main Authors: Leal, Pablo P, Hurd, Catriona L, Fernández, Pamela A, Roleda, Michael Y
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2017
• Subjects: Benthos; Bottles or small containers/Aquaria <20 L; Chromista; Coast and continental shelf; Laboratory experiment; Macroalgae; Macrocystis pyrifera; Ochrophyta; Reproduction; FOS Medical biotechnology; Single species; South Pacific; Temperate; Undaria pinnatifida; Type; Species; Registration number of species; Uniform resource locator/link to reference; Experiment duration; Temperature, water; Treatment; Germination rate; Germination rate, standard deviation; Growth rate; Growth rate, standard deviation; Gametophyte size; Gametophyte size, standard deviation; Ratio; Ratio, standard deviation; Salinity; pH; pH, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Bicarbonate ion; Bicarbonate ion, standard deviation; Carbon dioxide; Carbon dioxide, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Carbonate system computation flag; Fugacity of carbon dioxide water at sea surface temperature wet air; Aragonite saturation state; Calcite saturation state; Experiment; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Hurd; Pablo; Pacific; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.875583; https://doi.pangaea.de/10.1594/PANGAEA.875583

Anthropogenic atmospheric emissions of CO2 are responsible for simultaneous ocean warming (OW) and ocean acidification (OA). These global events can have important impacts on marine fleshy macroalgae and coastal ecosystems. To understand the effects of OW and OA on the early life history stages of native (Macrocystis pyrifera) and invasive (Undaria pinnatifida) macroalgae, a multi-factorial experiment was performed to determine the independent and interactive effects of the drivers and the corresponding species-specific responses. Meiospores of M. pyrifera and U. pinnatifida were separately exposed to a 4 * 2 factorial design of seawater pH (pHT 7.20, extreme OA predicted for 2300; pHT 7.65, OA predicted for 2100; pHT 8.03, ambient pH; and pHT 8.40, pre-industrial pH) and temperature (12 °C, seasonal average temperature; and 16 °C, OW predicted for 2100). Over 15 days, different physiological parameters (i.e. meiospore germination, germling growth rate, gametophyte development and sex ratio) were measured. Reduced seawater pH and elevated temperature had independent and significant effects on developmental processes (germling growth rate, and male and female gametophyte sizes were independently greater under OA and OW conditions), but the interaction of the abiotic factors had no effect on any stage of meiospore development of either species. Despite some small differences between species (e.g. sex ratio), results of this experiment suggest that microscopic stages of the native M. pyrifera and the invasive U. pinnatifida will respond similarly to OA and OW. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2017-05-17.