Painted goby larvae under high-CO2 fail to recognize reef sounds, supplement to: Castro, Joana M; Amorim, M Clara P; Oliveira, A P; Gonçalves, Emanuel J; Munday, Philip L; Simpson, Stephen D; Faria, Ana M (2017): Painted goby larvae under high-CO2 fail to recognize reef sounds. PLoS ONE, 12(1), e0170838

• Main Authors: Castro, Joana M, Amorim, M Clara P, Oliveira, A P, Gonçalves, Emanuel J, Munday, Philip L, Simpson, Stephen D, Faria, Ana M
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2017
• Subjects: Animalia; Behaviour; Chordata; Coast and continental shelf; Containers and aquaria 20-1000 L or < 1 m**2; Laboratory experiment; Nekton; North Atlantic; Pelagos; Pomatoschistus pictus; Single species; Temperate; Type; Species; Registration number of species; Uniform resource locator/link to reference; Date; Experiment duration; Treatment; Comment; Time; Logarithm; Length, standard; pH; pH, standard deviation; Temperature, water; Temperature, water, standard deviation; Salinity; Salinity, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Carbon, inorganic, dissolved; Aragonite saturation state; Calcite saturation state; Experiment; Potentiometric; Potentiometric titration; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.875087; https://doi.pangaea.de/10.1594/PANGAEA.875087

Atmospheric CO2 levels have been increasing at an unprecedented rate due to anthropogenic activity. Consequently, ocean pCO2 is increasing and pH decreasing, affecting marine life, including fish. For many coastal marine fishes, selection of the adult habitat occurs at the end of the pelagic larval phase. Fish larvae use a range of sensory cues, including sound, for locating settlement habitat. This study tested the effect of elevated CO2 on the ability of settlement-stage temperate fish to use auditory cues from adult coastal reef habitats. Wild late larval stages of painted goby (Pomatoschistus pictus) were exposed to control pCO2 (532 µatm, pH 8.06) and high pCO2 (1503 µatm, pH 7.66) conditions, likely to occur in nearshore regions subjected to upwelling events by the end of the century, and tested in an auditory choice chamber for their preference or avoidance to nighttime reef recordings. Fish reared in control pCO2 conditions discriminated reef soundscapes and were attracted by reef recordings. This behaviour changed in fish reared in the high CO2 conditions, with settlement-stage larvae strongly avoiding reef recordings. This study provides evidence that ocean acidification might affect the auditory responses of larval stages of temperate reef fish species, with potentially significant impacts on their survival. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2017-05-08.