An ocean acidification acclimatised green tide alga is robust to changes of seawater carbon chemistry but vulnerable to light stress, supplement to: Gao, Guang; Liu, Yameng; Li, Xinshu; Feng, Zhihua; Xu, Juntian; Lin, Y S (2016): An ocean acidification acclimatised green tide alga is robust to changes of seawater carbon chemistry but vulnerable to light stress. PLoS ONE, 11(12), e0169040

• Main Authors: Gao, Guang, Liu, Yameng, Li, Xinshu, Feng, Zhihua, Xu, Juntian, Lin, Y S
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2016
• Subjects: Benthos; Bottles or small containers/Aquaria <20 L; Chlorophyta; Coast and continental shelf; Growth/Morphology; Laboratory experiment; Light; Macroalgae; North Pacific; Plantae; Primary production/Photosynthesis; Single species; Temperate; Ulva linza; Type; Species; Registration number of species; Uniform resource locator/link to reference; Figure; Treatment; Net photosynthesis rate; Net photosynthesis rate, standard deviation; Growth rate; Growth rate, standard deviation; pH; Electron transport rate, relative; Electron transport rate, relative, standard deviation; Non photochemical quenching; Non photochemical quenching, standard deviation; Chlorophyll a; Chlorophyll a, standard deviation; Chlorophyll b; Chlorophyll b, standard deviation; Salinity; Temperature, water; pH, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Bicarbonate ion; Bicarbonate ion, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbon dioxide; Carbon dioxide, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Carbonate system computation flag; Fugacity of carbon dioxide water at sea surface temperature wet air; Aragonite saturation state; Calcite saturation state; Experiment; Potentiometric; Calculated using CO2SYS; Potentiometric titration; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Pacific; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.874786; https://doi.pangaea.de/10.1594/PANGAEA.874786

Ulva is the dominant genus in the green tide events and is considered to have efficient CO2 concentrating mechanisms (CCMs). However, little is understood regarding the impacts of ocean acidification on the CCMs of Ulva and the consequences of thalli's acclimation to ocean acidification in terms of responding to environmental factors. Here, we grew a cosmopolitan green alga, Ulva linza at ambient (LC) and elevated (HC) CO2 levels and investigated the alteration of CCMs in U. linza grown at HC and its responses to the changed seawater carbon chemistry and light intensity. The inhibitors experiment for photosynthetic inorganic carbon utilization demonstrated that acidic compartments, extracellular carbonic anhydrase (CA) and intracellular CA worked together in the thalli grown at LC and the acquisition of exogenous carbon source in the thalli could be attributed to the collaboration of acidic compartments and extracellular CA. Contrastingly, when U. linza was grown at HC, extracellular CA was completely inhibited, acidic compartments and intracellular CA were also down-regulated to different extents and thus the acquisition of exogenous carbon source solely relied on acidic compartments. The down-regulated CCMs in U. linza did not affect its responses to changes of seawater carbon chemistry but led to a decrease of net photosynthetic rate when thalli were exposed to increased light intensity. This decrease could be attributed to photodamage caused by the combination of the saved energy due to the down-regulated CCMs and high light intensity. Our findings suggest future ocean acidification might impose depressing effects on green tide events when combined with increased light exposure. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2017-04-21.