Low-steady-state metabolism induced by elevated CO2 increases resilience to UV radiation in the unicellular green-algae Dunaliella tertiolecta, supplement to: García-Gómez, Candela; Mata, M T; Van Breusegem, Frank; Segovia, María (2016): Low-steady-state metabolism induced by elevated CO2 increases resilience to UV radiation in the unicellular green-algae Dunaliella tertiolecta. Environmental and Experimental Botany, 132, 163-174

• Main Authors: García-Gómez, Candela, Mata, M T, Van Breusegem, Frank, Segovia, María
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2016
• Subjects: Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria <20 L; Chlorophyta; Dunaliella tertiolecta; Gene expression incl. proteomics; Laboratory experiment; Laboratory strains; Light; Not applicable; Pelagos; Phytoplankton; Plantae; Primary production/Photosynthesis; Single species; Type; Species; Registration number of species; Uniform resource locator/link to reference; Identification; Treatment; Time in days; Percentage; Cell density; Maximum photochemical quantum yield of photosystem II; Fluorescence; Reactive oxygen species production; Gross carbon fixation rate per cell; Gene expression; Salinity; Temperature, water; Initial slope of the photosynthesis-irradiance curve; Initial slope of rapid light curve, standard deviation; Maximal electron transport rate, relative; Maximal electron transport rate, relative, standard deviation; Light saturation; Light saturation, standard deviation; pH; pH, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Aragonite saturation state; Calcite saturation state; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Segovia; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.873652; https://doi.pangaea.de/10.1594/PANGAEA.873652

Global change factors derived from anthropogenic activities such as increased CO2 and ultraviolet radiation (UVR) have direct impacts on the physiological responses of organisms in both terrestrial and aquatic ecosystems. We assessed in this work the mechanistic, physiological and molecular response of the unicellular chlorophyte Dunaliella tertiolecta exposed to combinatorial present (390 ppmv, LC) and future CO2 levels predicted for the year 2100 (900 ppmv, HC) and different UVR doses. Growth rates, cell esterase activity, reactive oxygen species accumulation (ROS), and 14C fixation decreased under HC compared to LC. The deleterious effects of UVR were attenuated in HC, except for decreased photosynthetic capacities. Transcriptome analysis showed different expression patterns depending on UVR and CO2 levels. PsbA, LhcII and eCA gene expression was downregulated at HC compared to LC. However, photolyase (PL) gene expression was upregulated at HC with respect LC in UVR. This suggests that HC (as opposed to LC) promoted a "low-metabolic steady state", decreasing UVR exposure-activated repair by means of protein replacement. Only PL photoreactivation was active under HC. Our data add evidence on D. tertiolecta possibly becoming more resilient to UVR exposure under future CO2 regimes, and maybe other marine unicellular chlorophytes, warranting serious considerations on the consequent ecological implications. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2017-03-16.