A longitudinal study of Pacific oyster (Crassostrea gigas) larval development: isotope shifts during early shell formation reveal sub-lethal energetic stress ...

• Main Authors: Brunner, Elizabeth L, Prahl, Frederick G, Hales, Burke, Waldbusser, George G
• Format: Dataset
• Language: English
• Published: PANGAEA 2016
• Subjects: Animalia; Biomass/Abundance/Elemental composition; Coast and continental shelf; Crassostrea gigas; Growth/Morphology; Laboratory experiment; Mesocosm or benthocosm; Mollusca; North Pacific; Pelagos; Single species; Temperate; Zooplankton; Type; Species; Registration number of species; Uniform resource locator/link to reference; Experiment; Time in days; Fraction; Length; Length, standard deviation; Dry mass per individual; Lipids per individual; Proportion; Carbon/Nitrogen ratio; Calcification rate, mass normalized; δ13C; δ15N; δ13C, dissolved inorganic carbon; Temperature, water; Temperature, water, standard deviation; Salinity; Salinity, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; pH; pH, standard deviation; Aragonite saturation state; Aragonite saturation state, standard error; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Calcite saturation state; Pacific; Ocean acidification; Pacific oyster
• Online Access: https://dx.doi.org/10.1594/pangaea.873310; https://doi.pangaea.de/10.1594/PANGAEA.873310

Three cohorts of Pacific oyster (Crassostrea gigas) larvae at Whiskey Creek Shellfish Hatchery (WCH) in Netarts Bay, Oregon, were monitored for stable isotope incorporation and biochemical composition: one in May 2011 and two in August 2011. Along with measures of growth and calcification, we present measurements of stable isotopes of carbon in water, algal food, and the shell and tissue, and nitrogen in food and tissue across larval development and growth. These relatively unique measures through larval ontogeny allow us to document isotopic shifts associated with initiation and rate of feeding, and the catabolism of C-rich (lipid) and N-rich (protein) pools. Similar ontological patterns in growth and bulk composition among the cohorts reinforce prior results, suggesting that the creation of the initial shell is energetically expensive, that the major carbon source is ambient dissolved inorganic carbon, and that the major energetic source during this period is maternally derived egg lipids. The May cohort ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2017-03-07. ...