Metabolic responses to high pCO2 conditions at a CO2 vent site in juveniles of a marine isopod species assemblage, supplement to: Turner, Lucy M; Ricevuto, Elena; Gallucci, Alexia Massa; Lorenti, Maurizio; Gambi, Maria Cristina; Calosi, Piero (2016): Metabolic responses to high pCO2 conditions at a CO2 vent site in juveniles of a marine isopod species assemblage. Marine Biology, 163(10)

• Main Authors: Turner, Lucy M, Ricevuto, Elena, Gallucci, Alexia Massa, Lorenti, Maurizio, Gambi, Maria Cristina, Calosi, Piero
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2016
• Subjects: Animalia; Arthropoda; Benthic animals; Benthos; Bottles or small containers/Aquaria <20 L; CO2 vent; Coast and continental shelf; Cymodoce truncata; Dynamene bifida; Dynamene torelliae; Field experiment; Mediterranean Sea; Other metabolic rates; Single species; Temperate; Event label; Type; Species; Registration number of species; Uniform resource locator/link to reference; Treatment; Adenosine triphosphate, per unit protein; Adenosine 5-Triphosphate, standard error; Replicates; L-lactate; L-lactate, standard error; Carbonic anhydrase activity, per protein; Carbonic anhydrase activity, standard error; Salinity; Salinity, standard error; Temperature, water; Temperature, water, standard error; pH; pH, standard error; Alkalinity, total; Alkalinity, total, standard error; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard error; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide water at sea surface temperature wet air, standard error; Bicarbonate ion; Bicarbonate ion, standard error; Carbonate ion; Carbonate ion, standard error; Calcite saturation state; Calcite saturation state, standard error; Aragonite saturation state; Aragonite saturation state, standard error; Station label; Survival; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Experiment; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.873309; https://doi.pangaea.de/10.1594/PANGAEA.873309

We are starting to understand the relationship between metabolic rate responses and species' ability to respond to exposure to high pCO2. However, most of our knowledge has come from investigations of single species. The examination of metabolic responses of closely related species with differing distributions around natural elevated CO2 areas may be useful to inform our understanding of their adaptive significance. Furthermore, little is known about the physiological responses of marine invertebrate juveniles to high pCO2, despite the fact they are known to be sensitive to other stressors, often acting as bottlenecks for future species success. We conducted an in situ transplant experiment using juveniles of isopods found living inside and around a high pCO2 vent (Ischia, Italy): the CO2 'tolerant' Dynamene bifida and 'sensitive' Cymodoce truncata and Dynamene torelliae. This allowed us to test for any generality of the hypothesis that pCO2 sensitive marine invertebrates may be those that experience trade-offs between energy metabolism and cellular homoeostasis under high pCO2 conditions. Both sensitive species were able to maintain their energy metabolism under high pCO2 conditions, but in C. truncata this may occur at the expense of [carbonic anhydrase], confirming our hypothesis. By comparison, the tolerant D. bifida appeared metabolically well adapted to high pCO2, being able to upregulate ATP production without recourse to anaerobiosis. These isopods are important keystone species; however, given they differ in their metabolic responses to future pCO2, shifts in the structure of the marine ecosystems they inhabit may be expected under future ocean acidification conditions. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2017-03-07.