Sea hare Aplysia punctata (mollusca: Gastropoda) can maintain shell calcification under extreme ocean acidification ...

• Main Authors: Carey, Nicholas, Dupont, Sam, Sigwart, Julia D
• Format: Dataset
• Language: English
• Published: PANGAEA 2016
• Subjects: Animalia; Aplysia punctata; Benthic animals; Benthos; Calcification/Dissolution; Coast and continental shelf; Containers and aquaria 20-1000 L or < 1 m**2; Growth/Morphology; Laboratory experiment; Mollusca; North Atlantic; Respiration; Single species; Temperate; Type; Species; Registration number of species; Uniform resource locator/link to reference; Sample ID; Treatment; Dry mass; Shell length; Width; Respiration rate, oxygen; Temperature, water; Temperature, water, standard deviation; pH, total scale; pH, standard deviation; Salinity; Salinity, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Calcite saturation state; Calcite saturation state, standard deviation; Aragonite saturation state; Aragonite saturation state, standard deviation; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Potentiometric; Potentiometric titration; Calculated using CO2calc; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.872400; https://doi.pangaea.de/10.1594/PANGAEA.872400

Ocean acidification is expected to cause energetic constraints upon marine calcifying organisms such as molluscs and echinoderms, because of the increased costs of building or maintaining shell material in lower pH. We examined metabolic rate, shell morphometry, and calcification in the sea hare Aplysia punctata under short-term exposure (19 days) to an extreme ocean acidification scenario (pH 7.3, 2800 µatm pCO2), along with a group held in control conditions (pH 8.1, 344 µatm pCO2). This gastropod and its congeners are broadly distributed and locally abundant grazers, and have an internal shell that protects the internal organs. Specimens were examined for metabolic rate via closed-chamber respirometry, followed by removal and examination of the shell under confocal microscopy. Staining using calcein determined the amount of new calcification that occurred over 6 days at the end of the acclimation period. The width of new, pre-calcified shell on the distal shell margin was also quantified as a proxy for ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2017-02-20. ...