Hyperventilation and blood acid-base balance in hypercapnia exposed red drum (Sciaenops ocellatus), supplement to: Ern, Rasmus; Esbaugh, Andrew J (2016): Hyperventilation and blood acid–base balance in hypercapnia exposed red drum (Sciaenops ocellatus). Journal of Comparative Physiology B-Biochemical Systemic and Environmentalphysiology, 186(4), 447-460

• Main Authors: Ern, Rasmus, Esbaugh, Andrew J
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2016
• Subjects: Animalia; Behaviour; Chordata; Coast and continental shelf; Containers and aquaria 20-1000 L or < 1 m**2; Laboratory experiment; Nekton; North Atlantic; Pelagos; Sciaenops ocellatus; Single species; Temperate; Type; Species; Registration number of species; Uniform resource locator/link to reference; Treatment; Time in hours; Ventilation frequency; Ventilation frequency, standard error; Ventilation amplitude, relative; Ventilation amplitude, relative, standard error; Ventilation stroke volume, relative; Ventilation stroke volume, relative, standard error; Ventilatory minute volume, relative; Ventilatory minute volume, relative, standard error; pH; pH, standard error; Carbon dioxide, partial pressure, blood; Carbon dioxide, partial pressure, blood, standard error; Carbon dioxide; Carbon dioxide, standard error; Salinity; Temperature, water; Temperature, water, standard error; Alkalinity, total; Alkalinity, total, standard error; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide water at sea surface temperature wet air, standard error; Carbonate system computation flag; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Carbon, inorganic, dissolved; Aragonite saturation state; Calcite saturation state; Potentiometric; Potentiometric titration; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification; Red drum
• Online Access: https://dx.doi.org/10.1594/pangaea.869471; https://doi.pangaea.de/10.1594/PANGAEA.869471

Hyperventilation is a common response in fish exposed to elevated water CO2. It is believed to lessen the respiratory acidosis associated with hypercapnia by lowering arterial PCO2, but the contribution of hyperventilation to blood acid-base compensation has yet to be quantified. Hyperventilation may also increase the flux of irons across the gill epithelium and the cost of osmoregulation, owing to the osmo-respiratory compromise. Therefore, hypercapnia exposed fish may increase standard metabolic rate (SMR) leaving less energy for physiological functions such as foraging, migration, growth and reproduction. Here we show that gill ventilation, blood PCO2 and total blood [CO2] increased in red drum (Sciaenops ocellatus) exposed to 1000 and 5000 µatm water CO2, and that blood PCO2 and total blood [CO2] decrease in fish during hypoxia induced hyperventilation. Based on these results we estimate the ventilatory contributions to total acid-base compensation in 1000 and 5000 µatm water CO2. We find that S. ocellatus only utilize a portion of its ventilatory capacity to reduce the acid-base disturbance in 1000 µatm water CO2. SMR was unaffected by both salinity and hypercapnia exposure indicating that the cost of osmoregulation is small relative to SMR, and that the lack of increased ventilation in 1000 µatm water CO2 despite the capacity to do so is not due to an energetic tradeoff between acid-base balance and osmoregulation. Therefore, while ocean acidification may impact ventilatory parameters, there will be little impact on the overall energy budget of S. ocellatus. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2016-12-13.