Juvenile pen shells (Pinna nobilis) tolerate acidification but are vulnerable to warming, supplement to: Basso, L; Hendriks, Iris; Duarte, Carlos Manuel (2015): Juvenile pen shells (Pinna nobilis) tolerate acidification but are vulnerable to warming. Estuaries and Coasts, 38(6), 1976-1985

• Main Authors: Basso, L, Hendriks, Iris, Duarte, Carlos Manuel
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2015
• Subjects: Animalia; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria 20-1000 L or < 1 m**2; Growth/Morphology; Laboratory experiment; Mediterranean Sea; Mollusca; Mortality/Survival; Pinna nobilis; Respiration; Single species; Temperate; Temperature; Type; Species; Registration number of species; Uniform resource locator/link to reference; Figure; Partial pressure of carbon dioxide water at sea surface temperature wet air; Temperature, water; Mortality; Mortality, standard deviation; Mortality, standard error; Growth rate; Growth rate, standard deviation; Respiration rate, oxygen; Respiration rate, oxygen, standard deviation; Salinity; Salinity, standard error; Temperature, water, standard error; pH; pH, standard error; Alkalinity, total; Alkalinity, total, standard error; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air, standard error; Bicarbonate ion; Bicarbonate, standard deviation; Carbonate ion; Carbonate ion, standard error; Calcite saturation state; Calcite saturation state, standard error; Aragonite saturation state; Aragonite saturation state, standard error; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Experiment; Spectrophotometric; Potentiometric titration; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Basso; Duarte; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.869318; https://doi.pangaea.de/10.1594/PANGAEA.869318

In the course of this century, rising anthropogenic CO2 emissions will likely cause a decrease in ocean pH, know as ocean acidification, together with an increase of water temperature. Only in the last years, studies have focused on synergetic effects of both stressors on marine invertebrates, particularly on early life stages considered more vulnerable. Disparate responses of their singular and combined effects were reported, highlighting the importance of extending the studies to different species and populations of marine invertebrates. Here, we observed the response of important parameters such as growth, mortality and oxygen consumption of juvenile pen shell Pinna nobilis at supplied pCO2 gas levels of 400 ppm (ambient) and 1000 ppm and at three temperatures (20, 23 and 26 °C) during 36 days. To our knowledge, this is the first study on ocean acidification and temperature effects on juveniles of this species. We show that the two stressors play roles at distinct levels, with pCO2 influencing growth and partially mortality, and temperature increasing mortality rates and oxygen consumption strongly. Therefore, juveniles of P. nobilis are more likely affected by increasing temperature than the pCO2 levels expected by the end of the twenty-first century. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2016-12-12.