Interactive effects of ocean acidification and nitrogen limitation on two bloom-forming dinoflagellate species, supplement to: Eberlein, Tim; Van de Waal, Dedmer B; Brandenburg, Karen; John, Uwe; Voss, Maren; Achterberg, Eric Pieter; Rost, Björn (2016): Interactive effects of ocean acidification and nitrogen limitation on two bloom-forming dinoflagellate species. Marine Ecology Progress Series, 543, 127-140

• Main Authors: Eberlein, Tim, Van de Waal, Dedmer B, Brandenburg, Karen, John, Uwe, Voss, Maren, Achterberg, Eric Pieter, Rost, Björn
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2016
• Subjects: Alexandrium fundyense; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria <20 L; Chromista; Growth/Morphology; Immunology/Self-protection; Laboratory experiment; Laboratory strains; Macro-nutrients; Myzozoa; Not applicable; Pelagos; Phytoplankton; Scrippsiella trochoidea; Single species; Type; Species; Registration number of species; Uniform resource locator/link to reference; Replicate; Carbon, organic, particulate/Nitrogen, organic, particulate ratio; Carbon, organic, particulate, per cell; Nitrogen, organic, particulate, per cell; Chlorophyll a per cell; Carbon, organic, particulate per chlorophyll a; Cell biovolume; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Cellular paralytic shellfish toxin, total; Cellular paralytic shellfish toxin, total, standard deviation; Gonyautoxins 1/4; Gonyautoxins 1/4, standard deviation; Neurotoxin saxitoxin; Neurotoxin saxitoxin, standard deviation; Gonyautoxins 2/3; Gonyautoxins 2/3, standard deviation; Di-sulfated toxins C1+C2; Di-sulfated toxins C1+C2, standard deviation; Neosaxitoxin; Neosaxitoxin, standard deviation; Toxicity, cellular; Toxicity, cellular, standard deviation; Cell density; Cell density, standard deviation; Nitrogen, inorganic, dissolved; Nitrogen, inorganic, dissolved, standard deviation; Temperature, water; Salinity; Carbon, inorganic, dissolved; Alkalinity, total; pH; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Aragonite saturation state; Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Biological Impacts of Ocean Acidification BIOACID; Ocean Acidification International Coordination Centre OA-ICC; Maren; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.868682; https://doi.pangaea.de/10.1594/PANGAEA.868682

Global climate change involves an increase in oceanic CO2 concentrations as well as thermal stratification of the water column, thereby reducing nutrient supply from deep to surface waters. Changes in inorganic carbon (C) or nitrogen (N) availability have been shown to affect marine primary production, yet little is known about their interactive effects. To test for these effects, we conducted continuous culture experiments under N limitation and exposed the bloom-forming dinoflagellate species Scrippsiella trochoidea and Alexandrium fundyense (formerly A. tamarense) to CO2 partial pressures ( pCO2) ranging between 250 and 1000 µatm. Ratios of particulate organic carbon (POC) to organic nitrogen (PON) were elevated under N limitation, but also showed a decreasing trend with increasing pCO2. PON production rates were highest and affinities for dissolved inorganic N were lowest under elevated pCO2, and our data thus demonstrate a CO2-dependent trade-off in N assimilation. In A. fundyense, quotas of paralytic shellfish poisoning toxins were lowered under N limitation, but the offset to those obtained under N-replete conditions became smaller with increasing pCO2. Consequently, cellular toxicity under N limitation was highest under elevated pCO2. All in all, our observations imply reduced N stress under elevated pCO2, which we attribute to a reallocation of energy from C to N assimilation as a consequence of lowered costs in C acquisition. Such interactive effects of ocean acidification and nutrient limitation may favor species with adjustable carbon concentrating mechanisms and have consequences for their competitive success in a future ocean. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2016-11-22.