KOSMOS Finland 2012 mesocosm study: Size-fractionated bacterial protein production (BPP) of free-living and particle associated bacteria and abundance of particle associated heterotrophic prokaryotes. ...

• Main Authors: Hornick, Thomas, Bach, Lennart Thomas, Crawfurd, Katharine J, Spilling, Kristian, Achterberg, Eric Pieter, Woodhouse, Jason N, Schulz, Kai Georg, Brussaard, Corina P D, Riebesell, Ulf, Grossart, Hans-Peter
• Format: Dataset
• Language: English
• Published: PANGAEA 2016
• Subjects: DATE/TIME; Day of experiment; Mesocosm label; Protein production, particle associated bacteria; Protein production, free-living bacteria; Prokaryotes, heterotroph, particle associated; Mesocosm experiment; Biological Impacts of Ocean Acidification BIOACID; Surface Ocean Processes in the Anthropocene SOPRAN; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.868621; https://doi.pangaea.de/10.1594/PANGAEA.868621

The oceans absorb about a quarter of the annually produced anthropogenic atmospheric carbon dioxide (CO2), resulting in a decrease in surface water pH, a process termed ocean acidification (OA). Surprisingly little is known about how OA affects the physiology of heterotrophic bacteria or the coupling of heterotrophic bacteria to phytoplankton when nutrients are limited. Previous experiments were, for the most part, undertaken during productive phases or following nutrient additions designed to stimulate algal blooms. Therefore, we performed an in situ large-volume mesocosm (ca. 55 m**3) experiment in the Baltic Sea by simulating different fugacities of CO2 (fCO2) extending from present to future conditions. The study was conducted in July?August after the nominal spring bloom, in order to maintain low-nutrient conditions throughout the experiment. This resulted in phytoplankton communities dominated by small-sized functional groups (picophytoplankton). There was no consistent fCO2-induced effect on bacterial ... : Bacterial protein production was determined by measuring incorporation of 14C-Leucine.Abundance of particle associated heterotrophic prokaryotes was determined by epifluorescence microscopy. ...