A direct CO2 control system for ocean acidification experiments: testing effects on the coralline red algae Phymatolithon lusitanicum, supplement to: Sordo, Laura; Santos, Rui; Reis, Joao; Shulika, Alona; Silva, João (2016): A direct CO2 control system for ocean acidification experiments: testing effects on the coralline red algae Phymatolithon lusitanicum. PeerJ, 4, e2503
Most ocean acidification (OA) experimental systems rely on pH as an indirect way to control CO2. However, accurate pH measurements are difficult to obtain and shifts in temperature and/or salinity alter the relationship between pH and pCO2. Here we describe a system in which the target pCO2 is contr...
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Format: | Dataset |
Language: | English |
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PANGAEA - Data Publisher for Earth & Environmental Science
2016
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Subjects: | |
Online Access: | https://dx.doi.org/10.1594/pangaea.867506 https://doi.pangaea.de/10.1594/PANGAEA.867506 |
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ftdatacite:10.1594/pangaea.867506 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
English |
topic |
Benthos Coast and continental shelf Containers and aquaria 20-1000 L or < 1 m**2 Laboratory experiment Macroalgae North Atlantic Phymatolithon lusitanicum Plantae Primary production/Photosynthesis Respiration Rhodophyta Single species Temperate Temperature Type Species Registration number of species Uniform resource locator/link to reference Partial pressure of carbon dioxide water at sea surface temperature wet air Irradiance Net photosynthesis rate, oxygen Temperature, water Respiration rate, oxygen Respiration rate, oxygen, standard error Alkalinity, total Alkalinity, total, standard error Salinity Salinity, standard error Temperature, water, standard error pH pH, standard error Carbon, inorganic, dissolved Carbon, inorganic, dissolved, standard error Aragonite saturation state Aragonite saturation state, standard error Partial pressure of carbon dioxide water at sea surface temperature wet air, standard error Carbonate system computation flag Carbon dioxide Fugacity of carbon dioxide water at sea surface temperature wet air Bicarbonate ion Carbonate ion Calcite saturation state Experiment Potentiometric titration Potentiometric Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. 2010 Ocean Acidification International Coordination Centre OA-ICC |
spellingShingle |
Benthos Coast and continental shelf Containers and aquaria 20-1000 L or < 1 m**2 Laboratory experiment Macroalgae North Atlantic Phymatolithon lusitanicum Plantae Primary production/Photosynthesis Respiration Rhodophyta Single species Temperate Temperature Type Species Registration number of species Uniform resource locator/link to reference Partial pressure of carbon dioxide water at sea surface temperature wet air Irradiance Net photosynthesis rate, oxygen Temperature, water Respiration rate, oxygen Respiration rate, oxygen, standard error Alkalinity, total Alkalinity, total, standard error Salinity Salinity, standard error Temperature, water, standard error pH pH, standard error Carbon, inorganic, dissolved Carbon, inorganic, dissolved, standard error Aragonite saturation state Aragonite saturation state, standard error Partial pressure of carbon dioxide water at sea surface temperature wet air, standard error Carbonate system computation flag Carbon dioxide Fugacity of carbon dioxide water at sea surface temperature wet air Bicarbonate ion Carbonate ion Calcite saturation state Experiment Potentiometric titration Potentiometric Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. 2010 Ocean Acidification International Coordination Centre OA-ICC Sordo, Laura Santos, Rui Reis, Joao Shulika, Alona Silva, João A direct CO2 control system for ocean acidification experiments: testing effects on the coralline red algae Phymatolithon lusitanicum, supplement to: Sordo, Laura; Santos, Rui; Reis, Joao; Shulika, Alona; Silva, João (2016): A direct CO2 control system for ocean acidification experiments: testing effects on the coralline red algae Phymatolithon lusitanicum. PeerJ, 4, e2503 |
topic_facet |
Benthos Coast and continental shelf Containers and aquaria 20-1000 L or < 1 m**2 Laboratory experiment Macroalgae North Atlantic Phymatolithon lusitanicum Plantae Primary production/Photosynthesis Respiration Rhodophyta Single species Temperate Temperature Type Species Registration number of species Uniform resource locator/link to reference Partial pressure of carbon dioxide water at sea surface temperature wet air Irradiance Net photosynthesis rate, oxygen Temperature, water Respiration rate, oxygen Respiration rate, oxygen, standard error Alkalinity, total Alkalinity, total, standard error Salinity Salinity, standard error Temperature, water, standard error pH pH, standard error Carbon, inorganic, dissolved Carbon, inorganic, dissolved, standard error Aragonite saturation state Aragonite saturation state, standard error Partial pressure of carbon dioxide water at sea surface temperature wet air, standard error Carbonate system computation flag Carbon dioxide Fugacity of carbon dioxide water at sea surface temperature wet air Bicarbonate ion Carbonate ion Calcite saturation state Experiment Potentiometric titration Potentiometric Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. 2010 Ocean Acidification International Coordination Centre OA-ICC |
description |
Most ocean acidification (OA) experimental systems rely on pH as an indirect way to control CO2. However, accurate pH measurements are difficult to obtain and shifts in temperature and/or salinity alter the relationship between pH and pCO2. Here we describe a system in which the target pCO2 is controlled via direct analysis of pCO2 in seawater. This direct type of control accommodates potential temperature and salinity shifts, as the target variable is directly measured instead of being estimated. Water in a header tank is permanently re-circulated through an air-water equilibrator. The equilibrated air is then routed to an infrared gas analyzer (IRGA) that measures pCO2 and conveys this value to a Proportional-Integral-Derivative (PID) controller. The controller commands a solenoid valve that opens and closes the CO2 flush that is bubbled into the header tank. This low-cost control system allows the maintenance of stabilized levels of pCO2 for extended periods of time ensuring accurate experimental conditions. This system was used to study the long term effect of OA on the coralline red algae Phymatolithon lusitanicum. We found that after 11 months of high CO2 exposure, photosynthesis increased with CO2 as opposed to respiration, which was positively affected by temperature. Results showed that this system is adequate to run long-term OA experiments and can be easily adapted to test other relevant variables simultaneously with CO2, such as temperature, irradiance and nutrients. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2015) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2016-11-03. |
format |
Dataset |
author |
Sordo, Laura Santos, Rui Reis, Joao Shulika, Alona Silva, João |
author_facet |
Sordo, Laura Santos, Rui Reis, Joao Shulika, Alona Silva, João |
author_sort |
Sordo, Laura |
title |
A direct CO2 control system for ocean acidification experiments: testing effects on the coralline red algae Phymatolithon lusitanicum, supplement to: Sordo, Laura; Santos, Rui; Reis, Joao; Shulika, Alona; Silva, João (2016): A direct CO2 control system for ocean acidification experiments: testing effects on the coralline red algae Phymatolithon lusitanicum. PeerJ, 4, e2503 |
title_short |
A direct CO2 control system for ocean acidification experiments: testing effects on the coralline red algae Phymatolithon lusitanicum, supplement to: Sordo, Laura; Santos, Rui; Reis, Joao; Shulika, Alona; Silva, João (2016): A direct CO2 control system for ocean acidification experiments: testing effects on the coralline red algae Phymatolithon lusitanicum. PeerJ, 4, e2503 |
title_full |
A direct CO2 control system for ocean acidification experiments: testing effects on the coralline red algae Phymatolithon lusitanicum, supplement to: Sordo, Laura; Santos, Rui; Reis, Joao; Shulika, Alona; Silva, João (2016): A direct CO2 control system for ocean acidification experiments: testing effects on the coralline red algae Phymatolithon lusitanicum. PeerJ, 4, e2503 |
title_fullStr |
A direct CO2 control system for ocean acidification experiments: testing effects on the coralline red algae Phymatolithon lusitanicum, supplement to: Sordo, Laura; Santos, Rui; Reis, Joao; Shulika, Alona; Silva, João (2016): A direct CO2 control system for ocean acidification experiments: testing effects on the coralline red algae Phymatolithon lusitanicum. PeerJ, 4, e2503 |
title_full_unstemmed |
A direct CO2 control system for ocean acidification experiments: testing effects on the coralline red algae Phymatolithon lusitanicum, supplement to: Sordo, Laura; Santos, Rui; Reis, Joao; Shulika, Alona; Silva, João (2016): A direct CO2 control system for ocean acidification experiments: testing effects on the coralline red algae Phymatolithon lusitanicum. PeerJ, 4, e2503 |
title_sort |
direct co2 control system for ocean acidification experiments: testing effects on the coralline red algae phymatolithon lusitanicum, supplement to: sordo, laura; santos, rui; reis, joao; shulika, alona; silva, joão (2016): a direct co2 control system for ocean acidification experiments: testing effects on the coralline red algae phymatolithon lusitanicum. peerj, 4, e2503 |
publisher |
PANGAEA - Data Publisher for Earth & Environmental Science |
publishDate |
2016 |
url |
https://dx.doi.org/10.1594/pangaea.867506 https://doi.pangaea.de/10.1594/PANGAEA.867506 |
genre |
North Atlantic Ocean acidification |
genre_facet |
North Atlantic Ocean acidification |
op_relation |
https://cran.r-project.org/package=seacarb https://dx.doi.org/10.7717/peerj.2503 https://cran.r-project.org/package=seacarb |
op_rights |
Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/legalcode CC-BY-3.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1594/pangaea.867506 https://doi.org/10.7717/peerj.2503 |
_version_ |
1766137283835068416 |
spelling |
ftdatacite:10.1594/pangaea.867506 2023-05-15T17:37:23+02:00 A direct CO2 control system for ocean acidification experiments: testing effects on the coralline red algae Phymatolithon lusitanicum, supplement to: Sordo, Laura; Santos, Rui; Reis, Joao; Shulika, Alona; Silva, João (2016): A direct CO2 control system for ocean acidification experiments: testing effects on the coralline red algae Phymatolithon lusitanicum. PeerJ, 4, e2503 Sordo, Laura Santos, Rui Reis, Joao Shulika, Alona Silva, João 2016 text/tab-separated-values https://dx.doi.org/10.1594/pangaea.867506 https://doi.pangaea.de/10.1594/PANGAEA.867506 en eng PANGAEA - Data Publisher for Earth & Environmental Science https://cran.r-project.org/package=seacarb https://dx.doi.org/10.7717/peerj.2503 https://cran.r-project.org/package=seacarb Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/legalcode CC-BY-3.0 CC-BY Benthos Coast and continental shelf Containers and aquaria 20-1000 L or < 1 m**2 Laboratory experiment Macroalgae North Atlantic Phymatolithon lusitanicum Plantae Primary production/Photosynthesis Respiration Rhodophyta Single species Temperate Temperature Type Species Registration number of species Uniform resource locator/link to reference Partial pressure of carbon dioxide water at sea surface temperature wet air Irradiance Net photosynthesis rate, oxygen Temperature, water Respiration rate, oxygen Respiration rate, oxygen, standard error Alkalinity, total Alkalinity, total, standard error Salinity Salinity, standard error Temperature, water, standard error pH pH, standard error Carbon, inorganic, dissolved Carbon, inorganic, dissolved, standard error Aragonite saturation state Aragonite saturation state, standard error Partial pressure of carbon dioxide water at sea surface temperature wet air, standard error Carbonate system computation flag Carbon dioxide Fugacity of carbon dioxide water at sea surface temperature wet air Bicarbonate ion Carbonate ion Calcite saturation state Experiment Potentiometric titration Potentiometric Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. 2010 Ocean Acidification International Coordination Centre OA-ICC Supplementary Dataset dataset Dataset 2016 ftdatacite https://doi.org/10.1594/pangaea.867506 https://doi.org/10.7717/peerj.2503 2021-11-05T12:55:41Z Most ocean acidification (OA) experimental systems rely on pH as an indirect way to control CO2. However, accurate pH measurements are difficult to obtain and shifts in temperature and/or salinity alter the relationship between pH and pCO2. Here we describe a system in which the target pCO2 is controlled via direct analysis of pCO2 in seawater. This direct type of control accommodates potential temperature and salinity shifts, as the target variable is directly measured instead of being estimated. Water in a header tank is permanently re-circulated through an air-water equilibrator. The equilibrated air is then routed to an infrared gas analyzer (IRGA) that measures pCO2 and conveys this value to a Proportional-Integral-Derivative (PID) controller. The controller commands a solenoid valve that opens and closes the CO2 flush that is bubbled into the header tank. This low-cost control system allows the maintenance of stabilized levels of pCO2 for extended periods of time ensuring accurate experimental conditions. This system was used to study the long term effect of OA on the coralline red algae Phymatolithon lusitanicum. We found that after 11 months of high CO2 exposure, photosynthesis increased with CO2 as opposed to respiration, which was positively affected by temperature. Results showed that this system is adequate to run long-term OA experiments and can be easily adapted to test other relevant variables simultaneously with CO2, such as temperature, irradiance and nutrients. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2015) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2016-11-03. Dataset North Atlantic Ocean acidification DataCite Metadata Store (German National Library of Science and Technology) |