Ocean acidification affects fish spawning but not paternity at CO2 seeps, supplement to: Milazzo, Marco; Cattano, Carlo; Alonzo, Suzanne H; Foggo, A; Gristina, Michele; Rodolfo-Metalpa, Riccardo; Sinopoli, Mauro; Spatafora, Davide; Stiver, Kelly A; Hall-Spencer, Jason M (2016): Ocean acidification affects fish spawning but not paternity at CO2 seeps. Proceedings of the Royal Society B-Biological Sciences, 283(1835), 20161021

• Main Authors: Milazzo, Marco, Cattano, Carlo, Alonzo, Suzanne H, Foggo, A, Gristina, Michele, Rodolfo-Metalpa, Riccardo, Sinopoli, Mauro, Spatafora, Davide, Stiver, Kelly A, Hall-Spencer, Jason M
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2016
• Subjects: Animalia; Behaviour; Chordata; CO2 vent; Coast and continental shelf; Field observation; Mediterranean Sea; Nekton; Pelagos; Reproduction; FOS Medical biotechnology; Single species; Symphodus ocellatus; Temperate; Event label; Type; Species; Registration number of species; Uniform resource locator/link to reference; Identification; Location; Treatment; Fish, standard length; Wet mass; Age; Number of individuals; Individuals, standard error; Replicates; Courtship; Courtship, standard error; Number; Number, standard error; Number of spawning events; Number of spawning events, standard error; Eggs area; Eggs area, standard error; Eggs; Eggs, standard error; Dominant male paternity; Dominant male paternity, standard error; Salinity; Salinity, standard error; Temperature, water; Temperature, water, standard error; pH; pH, standard error; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide water at sea surface temperature wet air, standard error; Alkalinity, total; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Carbon, inorganic, dissolved; Aragonite saturation state; Calcite saturation state; Experiment; Potentiometric; Calculated using CO2SYS; Potentiometric titration; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.867446; https://doi.pangaea.de/10.1594/PANGAEA.867446

Fish exhibit impaired sensory function and altered behaviour at levels of ocean acidification expected to occur owing to anthropogenic carbon dioxide emissions during this century. We provide the first evidence of the effects of ocean acidification on reproductive behaviour of fish in the wild. Satellite and sneaker male ocellated wrasse (Symphodus ocellatus) compete to fertilize eggs guarded by dominant nesting males. Key mating behaviours such as dominant male courtship and nest defence did not differ between sites with ambient versus elevated CO2 concentrations. Dominant males did, however, experience significantly lower rates of pair spawning at elevated CO2 levels. Despite the higher risk of sperm competition found at elevated CO2, we also found a trend of lower satellite and sneaker male paternity at elevated CO2. Given the importance of fish for food security and ecosystem stability, this study highlights the need for targeted research into the effects of rising CO2 levels on patterns of reproduction in wild fish. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2015) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2016-10-31.