Pteropod eggs released at high pCO2 lack resilience to ocean acidification, supplement to: Manno, C; Peck, Victoria L; Tarling, Geraint A (2016): Pteropod eggs released at high pCO2 lack resilience to ocean acidification. Scientific Reports, 6, 25752

• Main Authors: Manno, C, Peck, Victoria L, Tarling, Geraint A
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2016
• Subjects: Animalia; Antarctic; Bottles or small containers/Aquaria <20 L; Development; Laboratory experiment; Limacina helicina antarctica; Mollusca; Open ocean; Pelagos; Polar; Reproduction; FOS Medical biotechnology; Single species; Zooplankton; Type; Species; Registration number of species; Uniform resource locator/link to reference; Time point, descriptive; Treatment; Eggs; Egg ribbons, per individual; Egg size; Eggs, carbon content per egg; Egg stages; Temperature, water; Temperature, standard deviation; Salinity; Salinity, standard deviation; pH; pH, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Carbon dioxide, partial pressure, standard deviation; Aragonite saturation state; Aragonite saturation state, standard deviation; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Calcite saturation state; Experiment; Potentiometric; Potentiometric titration; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Scotia Sea; Antarc*; Antarctica; Limacina helicina; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.865341; https://doi.pangaea.de/10.1594/PANGAEA.865341

The effects of ocean acidification (OA) on the early recruitment of pteropods in the Scotia Sea, was investigated considering the process of spawning, quality of the spawned eggs and their capacity to develop. Maternal OA stress was induced on female pteropods (Limacina helicina antarctica) through exposure to present day pCO2 conditions and two potential future OA states (750 matm and 1200 matm). The eggs spawned from these females, both before and during their exposure to OA, were incubated themselves in this same range of conditions (embryonic OA stress). Maternal OA stress resulted in eggs with lower carbon content, while embryonic OA stress retarded development. The combination of maternal and embryonic OA stress reduced the percentage of eggs successfully reaching organogenesis by 80%. We propose that OA stress not only affects the somatic tissue of pteropods but also the functioning of their gonads. Corresponding in-situ sampling found that post-larval L. helicina antarctica concentrated around 600 m depth, which is deeper than previously assumed. A deeper distribution makes their exposure to waters undersaturated for aragonite more likely in the near future given that these waters are predicted to shoal from depth over the coming decades. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2015) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2016-09-12.