Contrasting effects of ocean acidification on reproduction in reef fishes, supplement to: Welch, Megan J; Munday, Philip L (2015): Contrasting effects of ocean acidification on reproduction in reef fishes. Coral Reefs, 35(2), 485-493

• Main Authors: Welch, Megan J, Munday, Philip L
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2016
• Subjects: Acanthochromis polyacanthus; Amphriprion percula; Animalia; Chordata; Coast and continental shelf; Containers and aquaria 20-1000 L or < 1 m**2; Growth/Morphology; Laboratory experiment; Mortality/Survival; Nekton; Pelagos; Reproduction; FOS Medical biotechnology; Single species; South Pacific; Tropical; Event label; Type; Species; Registration number of species; Uniform resource locator/link to reference; Treatment; Clutches per pair; Clutches per pair, standard error; Replicates; Eggs per clutch; Eggs per clutch, standard error; Eggs area; Eggs area, standard error; Survival; Survival rate, standard error; Egg hatching success; Egg hatching success, standard error; Length; Length, standard error; Mass; Mass, standard error; pH; pH, standard deviation; Temperature, water; Temperature, water, standard deviation; Salinity; Salinity, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Carbon, inorganic, dissolved; Aragonite saturation state; Calcite saturation state; Experiment; Potentiometric; Potentiometric titration; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Pacific; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.860455; https://doi.pangaea.de/10.1594/PANGAEA.860455

Differences in the sensitivity of marine species to ocean acidification will influence the structure of marine communities in the future. Reproduction is critical for individual and population success, yet is energetically expensive and could be adversely affected by rising CO2 levels in the ocean. We investigated the effects of projected future CO2 levels on reproductive output of two species of coral reef damselfish, Amphiprion percula and Acanthochromis polyacanthus. Adult breeding pairs were maintained at current-day control (446 µatm), moderate (652 µatm) or high CO2 (912 µatm) for a 9-month period that included the summer breeding season. The elevated CO2 treatments were consistent with CO2 levels projected by 2100 under moderate (RCP6) and high (RCP8) emission scenarios. Reproductive output increased in A. percula, with 45-75 % more egg clutches produced and a 47-56 % increase in the number of eggs per clutch in the two elevated CO2 treatments. In contrast, reproductive output decreased at high CO2 in Ac. polyacanthus, with approximately one-third as many clutches produced compared with controls. Egg survival was not affected by CO2 for A. percula, but was greater in elevated CO2 for Ac. polyacanthus. Hatching success was also greater for Ac. polyacanthus at elevated CO2, but there was no effect of CO2 treatments on offspring size. Despite the variation in reproductive output, body condition of adults did not differ between control and CO2 treatments in either species. Our results demonstrate different effects of high CO2 on fish reproduction, even among species within the same family. A greater understanding of the variation in effects of ocean acidification on reproductive performance is required to predict the consequences for future populations of marine organisms. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2015) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2016-05-06.