Combined effects of low pH and low oxygen on the early-life stages of the barnacle Balanus amphitrite, supplement to: Campanati, Camilla; Yip, Stella; Lane, Ackley Charles; Thiyagarajan, Vengatesen (2016): Combined effects of low pH and low oxygen on the early-life stages of the barnacle Balanus amphitrite. ICES Journal of Marine Science, 73(3), 791-802

• Main Authors: Campanati, Camilla, Yip, Stella, Lane, Ackley Charles, Thiyagarajan, Vengatesen
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2016
• Subjects: Animalia; Arthropoda; Balanus amphitriterite; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria <20 L; Coast and continental shelf; Development; Growth/Morphology; Laboratory experiment; North Pacific; Oxygen; Pelagos; Reproduction; FOS Medical biotechnology; Single species; Tropical; Zooplankton; Type; Species; Registration number of species; Uniform resource locator/link to reference; Treatment; Stage; Time in days; Percentage; Percentage, standard deviation; Percentage, standard error; Identification; Clearance rate per individual; Clearance rate, standard deviation; Lipids per individual; Settlement; Growth rate; Biomass, ash free dry mass per individual; Condition index; pH; Temperature, water; Salinity; Alkalinity, total; Carbon, inorganic, dissolved; Partial pressure of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Calcite saturation state; Aragonite saturation state; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Experiment; Potentiometric; Potentiometric titration; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Pacific; Stella; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.859435; https://doi.pangaea.de/10.1594/PANGAEA.859435

Ocean acidification (OA) is anticipated to interact with the more frequently occurring hypoxic conditions in shallow coastal environments. These could exert extreme stress on the barnacle-dominated fouling communities. However, the interactive effect of these two emerging stressors on early-life stages of fouling organisms remains poorly studied. We investigated both the independent and interactive effect of low pH (7.6 vs. ambient 8.2) and low oxygen (LO; 3 mg/l vs. ambient 5 mg/l) from larval development through settlement (attachment and metamorphosis) and juvenile growth of the widespread fouling barnacle, Balanus amphitrite. In particular, we focused on the critical transition between planktonic and benthic phases to examine potential limiting factors (i.e. larval energy storage and the ability to perceive cues) that may restrain barnacle recruitment under the interactive stressors. LO significantly slowed naupliar development, while the interaction with low pH (LO-LP) seemed to alleviate the negative effect. However, 20-50% of the larvae became cyprid within 4 d post-hatching, regardless of treatment. Under the two stressors interaction (LO-LP), the barnacle larvae increased their feeding rate, which may explain why their energy reserves at competency were not different from any other treatment. In the absence of a settlement-inducing cue, a significantly lower percentage of cyprids (15% lower) settled in LO and LO-LP. The presence of an inducing cue, however, elevated attachment up to 50-70% equally across all treatments. Post-metamorphic growth was not altered, although the condition index was different between LO and LO-LP treatments, potentially indicating that less and/or weaker calcified structures were developed when the two stressors were experienced simultaneously. LO was the major driver for the responses observed and its interaction with low pH should be considered in future studies to avoid underestimating the sensitivity of biofouling species to OA and associated climate change stressors. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2015) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2016-04-11.